Enhancing the in vitro anticancer activity of albendazole incorporated into chitosan-coated PLGA nanoparticles
- Authors
- Kang, Bong-Seok; Choi, Jin-Seok; Lee, Sang-Eun; Lee, Joon-Kyung; Kim, Tae-Hyeon; Jang, Woo Suk; Tunsirikongkon, Amolnat; Kim, Jin-Ki; Park, Jeong-Sook
- Issue Date
- Mar-2017
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Albendazole; PLGA Nanoparticle; Chitosan; Mucoadhesion; Anticancer activity
- Citation
- CARBOHYDRATE POLYMERS, v.159, pp.39 - 47
- Indexed
- SCIE
SCOPUS
- Journal Title
- CARBOHYDRATE POLYMERS
- Volume
- 159
- Start Page
- 39
- End Page
- 47
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/10098
- DOI
- 10.1016/j.carbpol.2016.12.009
- ISSN
- 0144-8617
- Abstract
- To improve the solubility and anticancer activity of albendazole (ABZ), chitosan (CS)-coated poly-dl-lactic-co-glycolic acid (PLGA) nanoparticles were developed. CS was used to coat ABZ-loaded PLGA nanoparticles to enhance both mucoadhesiveness and colloidal stability. CS-coated PLGA nanoparticles were prepared by suspending the nanoparticles in CS solution after solvent diffusion. The CS-coated PLGA nanoparticles were characterized, and ABZ release was studied in vitro from various formulations. The mucoadhesive properties and in vitro anticancer activities of CS-coated PLGA nanoparticles were investigated by measurement of zeta potentials and the MTT assay, respectively. Spherical nanoparticles below 500 nm in diameter were successfully prepared; the particle size distribution was narrow. Complete encapsulation of ABZ in CS-coated PLGA nanoparticles was confirmed by SEM, FTIR, DSC, and XRD. The particle sizes of CS-coated PLGA nanoparticles were in the range of 260-480 nm; the encapsulation efficiency was 43.4-54.6%; and the yield 58.5-67.8%. The zeta potential of CS-coated nanoparticles was above +27 mV and stability was maintained for 4 weeks. At pH 7.4, the in vitro release of ABZ from nanoparticles (P188-5) was 200-fold higher than that from untreated ABZ; this persisted for 12 h. Moreover, ABZ release from CS-coated PLGA nanoparticles (P188-CS0.5) was 1.5-fold higher than that from untreated ABZ at pH 1.2. Additionally, the ABZ-loaded CS-coated nanoparticles exhibited superior mucoadhesion and improved cytotoxicity. The results show that CS coating of PLGA nanoparticles may improve the anticancer effect and the mucoadhesive properties of ABZ-loaded nanoparticles. (C) 2016 Elsevier Ltd. All rights reserved.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF PHARMACY > DEPARTMENT OF PHARMACY > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.