Bioassay-Guided Separation ofCentipeda minimaUsing Comprehensive Linear Gradient Centrifugal Partition Chromatographyopen access
- Authors
- Kim, Ji Hoon; Jung, Eun Ju; Lee, Yun Jung; Gao, En Mei; Syed, Ahmed Shah; Kim, Chul Young
- Issue Date
- Jul-2020
- Publisher
- MDPI
- Keywords
- centrifugal partition chromatography; comprehensive linear gradient elution; bioactivity-guided isolation; 3-methoxyquercetin; brevilin A; Centipeda minima
- Citation
- MOLECULES, v.25, no.13, pp 1 - 9
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULES
- Volume
- 25
- Number
- 13
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/1024
- DOI
- 10.3390/molecules25133077
- ISSN
- 1420-3049
1420-3049
- Abstract
- A comprehensive linear gradient solvent system for centrifugal partition chromatography (CPC) was developed for the bioassay-guided isolation of natural compounds. The gradient solvent system consisted of three different ternary biphasic solvents types:n-hexane-acetonitrile-water (10:2:8,v/v), ethyl acetate-acetonitrile-water (10:2:8,v/v), and water-saturatedn-butanol-acetonitrile-water (10:2:8,v/v). The lower phase of then-hexane-acetonitrile-water (10:2:8,v/v) was used as the stationary phase, while its upper phase, as well as ethyl acetate-acetonitrile-water (10:2:8), and water-saturatedn-butanol-acetonitrile-water (10:2:8,v/v) were pumped to generate a linear gradient elution, increasing the mobile phase polarity. We used the gradient CPC to identify antioxidant response elements (AREs), inducing compounds fromCentipeda minima, using an ARE-luciferase assay in HepG2 cells, which led to the purification of the active molecules 3-methoxyquercetin and brevilin A. The developed CPC solvent systems allow the separation and isolation of compounds with a wide polarity range, allowing active molecule identification in the complex crude extract of natural products.
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