Chromofluorogenic naphthoquinolinedione-based probes for sensitive detection and removal of Hg2+ in aqueous solutions
- Authors
- Kumar, Ashwani; Hur, Won; Seong, Gi Hun; Kumar, Subodh; Chae, Pil Seok
- Issue Date
- Feb-2022
- Publisher
- Elsevier BV
- Keywords
- Naphthoquinolinedione; Hg2+/methylmercury detection; Fluorescence quenching; Naked eye detection; Bio-detection
- Citation
- Dyes and Pigments, v.198, pp 1 - 13
- Pages
- 13
- Indexed
- SCIE
SCOPUS
- Journal Title
- Dyes and Pigments
- Volume
- 198
- Start Page
- 1
- End Page
- 13
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/107960
- DOI
- 10.1016/j.dyepig.2021.110025
- ISSN
- 0143-7208
1873-3743
- Abstract
- Y Mercury ions are an industrial and environmental toxin that need to be monitored and regulated in aqueous samples. We prepared six probes (1-6) using a naphthoquinolinedione ring as a fluorophore platform that contains different substituents on the quinolone ring. All probes showed abilities to selectively bind Hg2+, but their sensitivity to the detection of this metal ion significantly varied depending on the substituent pattern. Among these probes, probe 5 with Br and CN substituents on the quinolone ring was most effective for the sensitive detection of Hg2+ in aqueous solutions. This probe showed a naked-eye color change from yellow to purple upon binding to Hg2+. A ratio-metric method using UV-visible absorption data gave a limit of detection (LOD) of 20 nM, while the fluorescence-based result yielded an LOD of 0.047 pM. When bio-samples such as human urine and serum were used as media, the fluorescence-based LOD of this probe for Hg2+ detection increased to 50 nM. Probe 5 was also effective at detecting methylmercury, an organic mercury species, dissolved in drainage water or urine. Based on the results of DLS, FE-SEM, and DFT calculations, the probe appeared to form large aggregates and undergo photo-induced electron transfer (PET) upon Hg2+ binding, both of which are responsible for the probe fluorescence quenching. Probe 5 also showed the ability to sense Hg2+ within three different types of human cells: Brain cells (U87MG), Hela cells and skin cancer cells (SK-MEL28). Furthermore, this probe holds significant potential for on-site applications since various solid supports such as silica gel, filter papers and TLC plates were effectively used for sensitive and selective Hg2+ sensing, following probe coating/adsorption.
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Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF BIONANO ENGINEERING > 1. Journal Articles
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