Preventive effects of the butanol fraction of Justicia procumbens L. against dexamethasone-induced muscle atrophy in C2C12 myotubesopen access
- Authors
- Kim, Jae -Yong; Kim, Hye Mi; Kim, Ji Hoon; Lee, Ju-Hee; Zhang, Kaixuan; Guo, Shuo; Lee, Do Hyun; Gao, Eun Mei; Son, Rak Ho; Kim, Seong-Min; Kim, Chul Young
- Issue Date
- Nov-2022
- Publisher
- Cell Press
- Keywords
- Justicia procumbens L; Muscle atrophy; Dexamethasone; Protein degradation
- Citation
- Heliyon, v.8, no.11, pp 1 - 9
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- Heliyon
- Volume
- 8
- Number
- 11
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/111572
- DOI
- 10.1016/j.heliyon.2022.e11597
- ISSN
- 2405-8440
- Abstract
- Skeletal muscle atrophy is associated with many diseases including cancer, inflammatory diseases, neuromuscular diseases, and acute critical illness. Justicia procumbens L. has been used as a herbal remedy, but the pharmaco-logical effect of J. procumbens on muscle atrophy has not yet been reported. Herein, we investigate the anti -atrophic effect of the n-butanol fraction of J. procumbens (JPBuFr) on dexamethasone (DEX)-induced muscle at-rophy in C2C12 myotubes. The myotubes diameter, MHC positive area, ROS production, and mitochondria contents were observed under a fluorescence microscope, and various proteins related to degradation or synthesis were analyzed by western blots. JPBuFr significantly attenuated a reduction of myotube diameter, mitochondrial content, ATP level, myosin heavy chain, and myogenin expression induced by DEX. Furthermore, co-treatment of DEX and JPBuFr not only increased phosphorylation of Akt, mTOR, and p70S6K proteins but also decreased reactive oxygen species production and expression of protein degradation factors (MuRF1, Atrogin-1, FoxO3a) compared to DEX treatment. These results suggest that JPBuFr may provide potential protective effects against muscle atrophy, giving it potential for the development of anti-atrophic health functional foods.
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