Inactivated Vibrio cholerae Strains That Express TcpA via the toxT-139F Allele Induce Antibody Responses against TcpA
- Authors
- Kim, Eun Jin; Bae, Jonghyun; Ju, Young-Jun; Ju, Do-Bin; Lee, Donghyun; Son, Seonghyeon; Choi, Hunseok; Ramamurthy, Thandavarayan; Yun, Cheol-Heui; Kim, Dong Wook
- Issue Date
- Nov-2022
- Publisher
- 한국미생물·생명공학회
- Keywords
- Cholera; toxin coregulated pilus (TCP); TCP major subunit A (TcpA); Vibrio cholerae; vaccine
- Citation
- Journal of Microbiology and Biotechnology, v.32, no.11, pp 1396 - 1405
- Pages
- 10
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 32
- Number
- 11
- Start Page
- 1396
- End Page
- 1405
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/112618
- DOI
- 10.4014/jmb.2209.09001
- ISSN
- 1017-7825
1738-8872
- Abstract
- Cholera remains a major global public health problem, for which oral cholera vaccines (OCVs) being a valuable strategy. Patients, who have recovered from cholera, develop antibody responses against LPS, cholera toxin (CT), toxin-coregulated pilus (TCP) major subunit A (TcpA) and other antigens; thus, these responses are potentially important contributors to immunity against Vibrio cholerae infection. However, assessments of the efficacy of current OCVs, especially inactivated OCVs, have focused primarily on O-antigen-specific antibody responses, suggesting that more sophisticated strategies are required for inactivated OCVs to induce immune responses against TCP, CT, and other antigens. Previously, we have shown that the toxT-139F allele enables V. cholerae strains to produce CT and TCP under simple laboratory culture conditions. Thus, we hypothesized that V. cholerae strains that express TCP via the toxT-139F allele induce TCP- specific antibody responses. As anticipated, V. cholerae strains that expressed TCP through the toxT- 139F allele elicited antibody responses against TCP when the inactivated bacteria were delivered via a mouse model. We have further developed TCP- expressing V. cholerae strains that have been used in inactivated OCVs and shown that they effect an antibody response against TcpA in vivo, suggesting that V. cholerae strains with the toxT-139F allele are excellent candidates for cholera vaccines.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF PHARMACY > DEPARTMENT OF PHARMACY > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/112618)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.