Freezing seawater for the long-term storage of bacterial cells for microscopic enumeration
- Authors
- Hyun, Jung-Ho; Yang, Eun-Jin
- Issue Date
- Sep-2003
- Publisher
- 한국미생물학회
- Keywords
- Freezing seawater; Microscopic enumeration; Storage of bacterial cells
- Citation
- The Journal of Microbiology, v.41, no.3, pp 262 - 265
- Pages
- 4
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- The Journal of Microbiology
- Volume
- 41
- Number
- 3
- Start Page
- 262
- End Page
- 265
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/112881
- ISSN
- 1225-8873
1976-3794
- Abstract
- Although enumerating bacterial cells is a fundamental step in understanding microbial ecosystems in marine environments, substantial decrease in bacterial counts with increasing sample storage time hampers the accurate estimation of bacterial biomass. We compared the variations in bacterial cell numbers caused by freezing and thawing of sample bottles or slides. Bacterial counts of seawater samples frozen only once in a sampling bottle yielded approximately 95% of the original numbers after 90 days, whereas 80% of the original count was obtained for samples prepared on slides. Only 67% and 58% of the original counts were recovered in samples repeatedly frozen and thawed in bottles or. on slides, respectively. The results indicated that freezing a seawater sample in a bottle increased the consistency of the epifluorescence microscopic enumeration of bacterial cells.
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Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > DEPARTMENT OF MARINE SCIENCE AND CONVERGENCE ENGINEERING > 1. Journal Articles
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