Lipidomic perturbations in lung, kidney, and liver tissues of p53 knockout mice analyzed by nanoflow UPLC-ESI-MS/MS
- Authors
- Park, Se Mi; Byeon, Seul Kee; Sung, Hyerim; Cho, Soo Young; Seong, Je Kyung; Moon, Myeong Hee
- Issue Date
- Oct-2016
- Publisher
- American Chemical Society
- Keywords
- kidney; lipidomic analysis; liver; lung; nUPLC-ESI-MS/MS; p53 knockout mouse
- Citation
- Journal of Proteome Research, v.15, no.10, pp 3763 - 3772
- Pages
- 10
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Journal of Proteome Research
- Volume
- 15
- Number
- 10
- Start Page
- 3763
- End Page
- 3772
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/114416
- ISSN
- 1535-3893
1535-3907
- Abstract
- Lipids are important signaling molecules regulating biological processes under normal and diseased conditions. Although p53 mutation is well-known for causing cancer, the relationship between p53-related tumorigenesis and altered lipid profile is unclear. We profiled differences in lipid expressions in liver, lung, and kidney in p53 knockout (KO) mice by high-speed quantitative analysis of 320 lipids (399 species identified) using nanoflow ultrahigh performance liquid chromatography-tandem mass spectrometry (nUPLC-MS/MS). Lung tissues were most severely affected by the lack of p53 gene, as shown by significant reduction (24-44%, P < 0.05) in total phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyelin (SM), diacylglycerol (DG), and triacylglycerol (TG), and significant increases (30-50%) in phosphatidylserine (PS), phosphatidylinositol (PI), and monohexosylceramide (MHC). MHC levels increased in all tissues. Dihexosylceramide (DHC) level decreased only in kidney tissue. Most PI, PS, and phosphatidic acid (PA) species showing significant increases contained a saturated acyl chain (18:0) in lung and liver tissues. Neutral glycerolipids (16:0/22:0-DG and most TGs with saturated and monounsaturated acyl chains) decreased 2-4-fold in the liver tissue. Our results suggest that the lack of p53 and altered lipid profiles are closely related, but as their changes vary from one tissue to another, the lipid alterations are tissue-specific. © 2016 American Chemical Society.
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