Rapid identification of methylglyoxal trapping constituents from onion peels by pre-column incubation method
- Authors
- Kim, Ji Hoon; Kim, Myeong Il; Syed, Ahmed Shah; Jung, Kiwon; Kim, Chul Young
- Issue Date
- Dec-2017
- Publisher
- Korean Society of Pharmacognosy
- Keywords
- Allium cepa L; Flavonoids; Methylglyoxal trapping; Pre-column incubation
- Citation
- Natural Product Sciences, v.23, no.4, pp.247 - 252
- Indexed
- SCOPUS
KCI
- Journal Title
- Natural Product Sciences
- Volume
- 23
- Number
- 4
- Start Page
- 247
- End Page
- 252
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/11621
- DOI
- 10.20307/nps.2017.23.4.247
- ISSN
- 1226-3907
- Abstract
- The methylglyoxal (MGO) trapping constituents from onion (Allium cepa L.) peels were investigated using pre-column incubation of MGO and crude extract followed by HPLC analysis. The peak areas of MGO trapping compounds decreased, and their chemical structures were identified by HPLC-ESI/MS. Among major constituents in outer scale of onion, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (2) was more effective MGO scavenger than quercetin (6) and its 4′-glucoside, spiraeoside (3). After 1 h incubation, compound 2 trapped over 90% MGO at a concentration of 0.5 mM under physiological conditions, but compounds 3 and 6 scavenged 45%, 16% MGO, respectively. HPLC-ESI/MS showed that compound 2 trapped two molecules of MGO to form a di-MGO adduct and compounds 3 and 6 captured one molecule of MGO to form mono-MGO adducts, and the positions 6 and 8 of the A ring of flavonoids were major active sites for trapping MGO. © 2017, Korean Society of Pharmacognosy. All rights reserved.
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