Differentiation of Scomber japonicus from Scomber scombrus by using a single locked nucleic acid probe
- Authors
- Ahn, Jeong Jin; Kim, Youngjoo; Hong, Ji Young; Kim, Gi Won; Kim, Seol Young; Hwang, Seung Yong
- Issue Date
- 2017
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Fluorescence melting curve analysis; Locked nucleic acid; Scomber japonicus; Scomber scombrus; species identification
- Citation
- Mitochondrial Dna Part a, v.28, no.3, pp.379 - 382
- Indexed
- SCIE
SCOPUS
- Journal Title
- Mitochondrial Dna Part a
- Volume
- 28
- Number
- 3
- Start Page
- 379
- End Page
- 382
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/11716
- DOI
- 10.3109/19401736.2015.1126831
- ISSN
- 2470-1394
- Abstract
- Mackerel is marketed at prices according to the species type, Scomber japonicus and Scomber scombrus. Distinguishing these two species with the naked eye is difficult, and their differentiation becomes more difficult after they are processed by cooking, thereby leading to counterfeiting issues. Thus, in this study, we developed a method to differentiate S. japonicus from S. scombrus by detecting polymorphisms in mitochondrial 16 s rRNA gene by using fluorescence melting curve analysis and locked nucleic acid probes. Our method could distinguish S. japonicus from S. scombrus in a single experiment by using a single probe. The probes developed matched exactly with S. japonicus and had a melting temperature of 64 degrees C. However, the probes were mismatched with S. scombrus, resulting in a lower melting temperature of 46 degrees C. The high specificity of the locked nucleic acid probes resulted in this large difference in the melting temperatures.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > ERICA 의약생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/11716)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.