Pyridoanthrone-based chromo-fluorogenic amphiphiles for selective CN- detection and their bioimaging application
- Authors
- Kumar, Ashwani; Vanita, Vanita; Walia, Amandeep; Chae, Pil Seok; Kumar, Subodh
- Issue Date
- Feb-2020
- Publisher
- Elsevier BV
- Keywords
- Chromofluorogenic; Pyridoanthrone-viologen amphiphiles; CN- sensor; Aqueous medium; Live cell imaging
- Citation
- Sensors and Actuators, B: Chemical, v.304, pp 1 - 15
- Pages
- 15
- Indexed
- SCIE
SCOPUS
- Journal Title
- Sensors and Actuators, B: Chemical
- Volume
- 304
- Start Page
- 1
- End Page
- 15
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/1295
- DOI
- 10.1016/j.snb.2019.127396
- ISSN
- 0925-4005
0925-4005
- Abstract
- Cyanide anion (CN-) is one of the most hazardous chemicals for humans but is inevitably used in various purposes. In this study, we prepared fluorescent pyridoanthrone-based probes 1 and 3 with an N-octyl viologen pendant to detect this toxic anion. Among various anions, the presence of CN- only turned over a large fluorescence enhancement of these viologen probes, giving an LOD of 1 and 10 nM for probes 1 and 3, respectively. These probes also enabled for 'naked-eye detection' thanks to the generation of vivid pink color in the presence of this anion. Other probes (2, 4 and 5) with no N-octyl viologen pendant were also capable of selectively sensing CN-, but clearly inferior to probes 1 and 3 in terms of detection sensitivity. The sensitive and fast CN- detection was ascribed to the efficient addition reaction of each N-octyl viologen probe with CN-, leading to the generation of a highly fluorescent hydrolyzed product, isolated as a pyridoanthrone amine derivative. Due to the presence of hydrophobic and aromatic-aromatic interactions as well as H-bonding between probe molecules, the probes organized into self-assemblies with the positive surfaces in aqueous solution, allowing fast addition reactions between the probe and CN- to produce color and fluorescence emission. Probe 1 proved effective at detecting CN- content in HeLa cells and could be used as a solid state for inexpensive and convenient detection of CN-. The current system was fully studied by various analytical methods such as UV-vis, fluorescence, and H-1 NMR spectroscopy, DLS, FE-SEM images, TEM, DFT calculations, HRMS and X-ray crystallography.
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