An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma
DC Field | Value | Language |
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dc.contributor.author | Rehman, Shaheed Ur | - |
dc.contributor.author | Choi, Min Sun | - |
dc.contributor.author | Kim, In Sook | - |
dc.contributor.author | Kim, Seung Hyun | - |
dc.contributor.author | Yoo, Hye Hyun | - |
dc.date.accessioned | 2021-06-22T16:21:41Z | - |
dc.date.available | 2021-06-22T16:21:41Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/13046 | - |
dc.description.abstract | Hederacoside C is a principal bioactive pharmaceutical ingredient of Hedera helix leaf extracts. H. helix extracts have long been used in folk medicine for the treatment of respiratory disorders. Currently, hederacoside C is investigated as a promising candidate for the treatment of respiratory diseases. In this study, an accurate, sensitive, rapid, and reliable bioanalytical method was developed for the determination of hederacoside C in rat plasma using ultra high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). For sample preparation, plasma proteins were precipitated with 0.1% acetic acid in acetonitrile. Waters UPLC BEH C-18 (2.1 mm I.D. x 100 mm, 1.7 mu m) column was used for chromatographic separation. A gradient elution of mobile phases consisting of 0.02% acetic acid in distilled water (solvent A) and 0.02% acetic acid in acetonitrile (solvent B) was used at a flow rate of 0.3 mL/min. The multiple reaction monitoring (MRM) mode was used for mass spectrometric detection; the MRM transitions were m/z 1219.7 -> m/z 469.2 for hederacoside C and m/z 1108.3 -> m/z 221.2 for ginsenoside Rb1 (internal standard) in the negative ionization mode. A calibration curve was constructed in the range of 10-1000 ng/mL. The intra- and inter-day precision and accuracy were within 5%. The developed UPLC-MS/MS method was successfully applied in a pharmacokinetic study of hederacoside C in rats. Hederacoside C was quickly but inadequately absorbed from the gastrointestinal tract of rats resulting in extremely low bioavailability and relatively slow clearance. (C) 2016 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | An ultra-high-performance liquid chromatography-tandem mass spectrometric method for the determination of hederacoside C, a drug candidate for respiratory disorder, in rat plasma | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoo, Hye Hyun | - |
dc.identifier.doi | 10.1016/j.jpba.2016.06.039 | - |
dc.identifier.scopusid | 2-s2.0-84978933356 | - |
dc.identifier.wosid | 000393847600012 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.129, pp.90 - 95 | - |
dc.relation.isPartOf | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | - |
dc.citation.title | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | - |
dc.citation.volume | 129 | - |
dc.citation.startPage | 90 | - |
dc.citation.endPage | 95 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | ALPHA-HEDERIN | - |
dc.subject.keywordPlus | HELIX | - |
dc.subject.keywordPlus | SAPONINS | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | LEAVES | - |
dc.subject.keywordPlus | PHARMACOKINETICS | - |
dc.subject.keywordPlus | HEDERAGENIN | - |
dc.subject.keywordPlus | FRUITS | - |
dc.subject.keywordAuthor | Hederacoside C | - |
dc.subject.keywordAuthor | Hedera helix | - |
dc.subject.keywordAuthor | UPLC-MS/MS | - |
dc.subject.keywordAuthor | Plasma | - |
dc.subject.keywordAuthor | Pharmacokinetics | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0731708516303569?via%3Dihub | - |
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