Circulating Gut-Homing (alpha(4)beta(+)(7)) Plasmablast Responses against Shigella Surface Protein Antigens among Hospitalized Patients with Diarrheaopen access
- Authors
- Sinha, Anuradha; Dey, Ayan; Saletti, Giulietta; Samanta, Pradip; Chakraborty, Partha Sarathi; Bhattacharya, M. K.; Ghosh, Santanu; Ramamurthy, T.; Kim, Jae-Ouk; Yang, Jae Seung; Kim, Dong Wook; Czerkinsky, Cecil; Nandy, Ranjan K.
- Issue Date
- Jul-2016
- Publisher
- AMER SOC MICROBIOLOGY
- Keywords
- ANTIBODY-FORMING-CELLS; MUCOSAL IMMUNE-RESPONSES; VACCINE DEVELOPMENT; DYSENTERIC PATIENTS; SECRETING CELLS; IMMUNOGENICITY; INFECTION; SONNEI; IMMUNIZATIONS
- Citation
- CLINICAL AND VACCINE IMMUNOLOGY, v.23, no.7, pp.610 - 617
- Indexed
- SCIE
SCOPUS
- Journal Title
- CLINICAL AND VACCINE IMMUNOLOGY
- Volume
- 23
- Number
- 7
- Start Page
- 610
- End Page
- 617
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/13212
- DOI
- 10.1128/CVI.00205-16
- ISSN
- 1556-6811
- Abstract
- Developing countries are burdened with Shigella diarrhea. Understanding mucosal immune responses associated with natural Shigella infection is important to identify potential correlates of protection and, as such, to design effective vaccines. We performed a comparative analysis of circulating mucosal plasmablasts producing specific antibodies against highly conserved invasive plasmid antigens (IpaC, IpaD20, and IpaD120) and two recently identified surface protein antigens, pan-Shigella surface protein antigen 1 (PSSP1) and PSSP2, common to all virulent Shigella strains. We examined blood and stool specimens from 37 diarrheal patients admitted to the Infectious Diseases & Beliaghata General Hospital, Kolkata, India. The etiological agent of diarrhea was investigated in stool specimens by microbiological methods and real-time PCR. Gut-homing (alpha(4)beta(+)(7)) antibody secreting cells (ASCs) were isolated from patient blood by means of combined magnetic cell sorting and two-color enzymelinked immunosorbent spot (ELISPOT) assay. Overall, 57% (21 of 37) and 65% (24 of 37) of the patients were positive for Shigella infection by microbiological and real-time PCR assays, respectively. The frequency of alpha(4)beta(+)(7) IgG ASC responders against Ipas was higher than that observed against PSSP1 or PSSP2, regardless of the Shigella serotype isolated from these patients. Thus, alpha(4)beta(+)(7) ASC responses to Ipas may be considered an indirect marker of Shigella infection. The apparent weakness of ASC responses to PSSP1 is consistent with the lack of cross-protection induced by natural Shigella infection. The finding that ASC responses to IpaD develop in patients with recent-onset shigellosis indicates that such responses may not be protective or may wane too rapidly and/or be of insufficient magnitude.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF PHARMACY > DEPARTMENT OF PHARMACY > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/13212)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.