Preparative separation of sesamin and sesamolin from defatted sesame meal via centrifugal partition chromatography with consecutive sample injection
- Authors
- Jeon, Je-Seung; Park, Chae Lee; Syed, Ahmed Shah; Kim, Young-Mi; Cho, Il Je; Kim, Chul Young
- Issue Date
- Feb-2016
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Centrifugal partition chromatography; Consecutive sample injection; Sesamin; Sesamolin; Antioxidant response element
- Citation
- JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.1011, pp.108 - 113
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
- Volume
- 1011
- Start Page
- 108
- End Page
- 113
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14535
- DOI
- 10.1016/j.jchromb.2015.12.062
- ISSN
- 1570-0232
- Abstract
- A preparative separation method using consecutive sample injection centrifugal partition chromatography (CPC) was developed to obtain sesamin and sesamolin from defatted sesame meal extracts. A two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water (8:2:8:2, viv) was applied in reversed-phase mode (descending mode). Preliminary experiments with an SCPC-100 (column volume: 100 mL) were performed to select the appropriate two-phase solvent system and sample injection times; these parameters were then used with an SCPC-1000 (column volume: 1000 mL) in a 10-fold scale-up preparative run. A sample containing 3 g of crude extract was consecutively injected four times onto the SCPC-1000, which yielded 328 mg of sesamin and 168 mg of sesamolin. These compounds were analyzed by high-performance liquid chromatography and determined to have purities of 95.6% and 93.9%, respectively. Sesamin and sesamolin (30 mu M) increased antioxidant response element (ARE) luciferase activity 2.6-fold and 1.9-fold, respectively. (C) 2016 Elsevier B.V. All rights reserved.
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