Two-stage, acetic acid-aqueous ammonia, fractionation of empty fruit bunches for increased lignocellulosic biomass utilization
- Authors
- Kim, Dong Young; Kim, Young Soo; Kim, Tae Hyun; Oh, Kyeong Keun
- Issue Date
- Jan-2016
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Weak acid; Weak alkali; Acetic acid hydrothermal (AAH); Pretreatment; Enzymatic digestibility
- Citation
- BIORESOURCE TECHNOLOGY, v.199, pp.121 - 127
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 199
- Start Page
- 121
- End Page
- 127
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14663
- DOI
- 10.1016/j.biortech.2015.09.049
- ISSN
- 0960-8524
- Abstract
- Fractionation of EFB was conducted in two consecutive steps using a batch reaction system: hemicellulose hydrolysis using acetic acid (AA; 3.0-7.0 wt.%) at 170-190 degrees C for 10-20 min in the first stage, and lignin solubilization using ammonium hydroxide (5-20 wt.%) at 140-220 degrees C for 5-25 min in the second stage. The two-stage process effectively fractionated empty fruit bunches (EFB) in terms of hemicellulose hydrolysis (53.6%) and lignin removal (59.5%). After the two-stage treatment, the fractionated solid contained 65.3% glucan. Among three investigated process parameters, reaction temperature and ammonia concentration had greater impact on the delignification reaction in the second stage than reaction time. The two-stage fractionation processing improved the enzymatic digestibility to 72.9% with 15 FPU of cellulase/g of glucan supplemented with 70 pNPG of beta-glycosidase (Novozyme 188)/g-glucan, which was significantly enhanced from the equivalent digestibility of 28.3% for untreated EFB and 45.7% for AAH-fractionated solid. (C) 2015 Elsevier Ltd. All rights reserved.
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