Cell-based microfluidic platform for mimicking human olfactory system
- Authors
- Lee, Seung Hwan; Oh, Eun-hae; Park, Tai Hyun
- Issue Date
- Dec-2015
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Cell-based odorant detection system; Gaseous odorant; Microfluidics platform; Olfactory receptor
- Citation
- BIOSENSORS & BIOELECTRONICS, v.74, pp 554 - 561
- Pages
- 8
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 74
- Start Page
- 554
- End Page
- 561
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/16135
- DOI
- 10.1016/j.bios.2015.06.072
- ISSN
- 0956-5663
1873-4235
- Abstract
- Various attempts have been made to mimic the human olfactory system using human olfactory receptors (hORs). In particular, OR-expressed cell-based odorant detection systems mimic the smell sensing mechanism of humans, as they exploit endogenous cellular signaling pathways. However, the majority of such cell-based studies have been performed in the liquid phase to maintain cell viability, and liquid odorants were used as detection targets. Here, we present a microfluidic device for the detection of gaseous odorants which more closely mimics the human olfactory system. Cells expressing hOR were cultured on a porous membrane. The membrane was then flipped over and placed between two compartments. The upper compartment is the gaseous part where gaseous odorants are supplied, while the lower compartment is the aqueous part where viable cells are maintained in the liquid medium. Using this simple microfluidic device, we were able to detect gaseous odorant molecules by a fluorescence signal. The fluorescence signal was generated by calcium influx resulting from the interaction between odorant molecules and the hOR. The system allowed detection of gaseous odorant molecules in real-time, and the findings showed that the fluorescence responses increased dose-dependently in the range of 0-2. ppm odorant. In addition, the system can discriminate among gaseous odorant molecules. This microfluidic system closely mimics the human olfactory system in the sense that the submerged cells detect gaseous odorants.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF BIONANO ENGINEERING > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/16135)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.