Highly sensitive immunoassay of anti-cyclic citrullinated peptide marker using surface-enhanced Raman scattering detection
DC Field | Value | Language |
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dc.contributor.author | Chon, Hyangah | - |
dc.contributor.author | Lee, Sangyeop | - |
dc.contributor.author | Wang,Rui | - |
dc.contributor.author | Bang, So-young | - |
dc.contributor.author | Lee, Hye-soon | - |
dc.contributor.author | Bae, Sang-cheol | - |
dc.contributor.author | Hong,Sung-hyun | - |
dc.contributor.author | Yoon, Young-ho | - |
dc.contributor.author | Lim, Dongwoo | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.accessioned | 2021-06-22T21:42:08Z | - |
dc.date.available | 2021-06-22T21:42:08Z | - |
dc.date.created | 2021-01-22 | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 1605-7422 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/20566 | - |
dc.description.abstract | We report a highly sensitive anti-cyclic citrullinated peptide (anti-CCP) detection method for early diagnosis of rheumatoid arthritis (RA) using surface-enhanced Raman scattering (SERS)-based immunoassay. Herein, cyclic citrullinated peptide (CCP)-conjugated magnetic beads and anti-human IgG-conjugated hollow gold nanospheres (HGNs) were used as substrates and SERS nano-tags, respectively. First, its detection sensitivity was evaluated using anti-CCP standard solutions. Then quantitative anti-CCP levels, determined by the SERS-based assay, were compared with those obtained from three commercially available anti-CCP assay kits (Immunoscan CCPlus, ImmunnLisa™ CCP and BioPlex™ 2200) to assess its potential utility as a clinical tool. Finally, clinical samples from 20 RA patients were investigated using them. In the SERS-based assay, the anti-CCP level in human serum was successfully determined by monitoring the characteristic Raman peak intensity of SERS nano-tags. The diagnostic performance of our SERS-based immunoassay for clinical samples shows a good agreement with those measured by three commercial anti-CCP kits. In addition, our SERS-based assay results are more consistent in the low concentration range (0-25 U/mL) than those achieved by the commercial kits. Accordingly, it is estimated that the SERS-based assay is a potentially useful diagnostic tool for early diagnosis of RA. © 2015 SPIE. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPIE | - |
dc.title | Highly sensitive immunoassay of anti-cyclic citrullinated peptide marker using surface-enhanced Raman scattering detection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Dongwoo | - |
dc.identifier.doi | 10.1117/12.2189760 | - |
dc.identifier.scopusid | 2-s2.0-84938718321 | - |
dc.identifier.wosid | 000359600900018 | - |
dc.identifier.bibliographicCitation | Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v.9523, pp.1 - 8 | - |
dc.relation.isPartOf | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | - |
dc.citation.title | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | - |
dc.citation.volume | 9523 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Conference Paper | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Optics | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Optics | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.subject.keywordPlus | Diagnosis | - |
dc.subject.keywordPlus | Diseases | - |
dc.subject.keywordPlus | Gold | - |
dc.subject.keywordPlus | Immunology | - |
dc.subject.keywordPlus | Nanospheres | - |
dc.subject.keywordPlus | Peptides | - |
dc.subject.keywordPlus | Raman scattering | - |
dc.subject.keywordPlus | Substrates | - |
dc.subject.keywordPlus | Blood samples | - |
dc.subject.keywordPlus | Cyclic-citrullinated peptides | - |
dc.subject.keywordPlus | Early diagnosis | - |
dc.subject.keywordPlus | Gold nanospheres | - |
dc.subject.keywordPlus | immunoassay | - |
dc.subject.keywordPlus | Magnetic beads | - |
dc.subject.keywordPlus | Rheumatoid arthritis | - |
dc.subject.keywordPlus | Surface enhanced Raman Scattering (SERS) | - |
dc.subject.keywordPlus | Surface scattering | - |
dc.subject.keywordAuthor | anti-cyclic citrullinated peptide antibody | - |
dc.subject.keywordAuthor | blood sample | - |
dc.subject.keywordAuthor | early diagnosis | - |
dc.subject.keywordAuthor | hollow gold nanosphere | - |
dc.subject.keywordAuthor | immunoassay | - |
dc.subject.keywordAuthor | magnetic bead | - |
dc.subject.keywordAuthor | rheumatoid arthritis | - |
dc.subject.keywordAuthor | Surface-enhanced Raman scattering | - |
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