Highly sensitive immunoassay of anti-cyclic citrullinated peptide marker using surface-enhanced Raman scattering detection
- Authors
- Chon, Hyangah; Lee, Sangyeop; Wang,Rui; Bang, So-young; Lee, Hye-soon; Bae, Sang-cheol; Hong,Sung-hyun; Yoon, Young-ho; Lim, Dongwoo; Choo, Jaebum
- Issue Date
- Feb-2015
- Publisher
- SPIE
- Keywords
- anti-cyclic citrullinated peptide antibody; blood sample; early diagnosis; hollow gold nanosphere; immunoassay; magnetic bead; rheumatoid arthritis; Surface-enhanced Raman scattering
- Citation
- Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v.9523, pp.1 - 8
- Indexed
- SCIE
SCOPUS
- Journal Title
- Progress in Biomedical Optics and Imaging - Proceedings of SPIE
- Volume
- 9523
- Start Page
- 1
- End Page
- 8
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/20566
- DOI
- 10.1117/12.2189760
- ISSN
- 1605-7422
- Abstract
- We report a highly sensitive anti-cyclic citrullinated peptide (anti-CCP) detection method for early diagnosis of rheumatoid arthritis (RA) using surface-enhanced Raman scattering (SERS)-based immunoassay. Herein, cyclic citrullinated peptide (CCP)-conjugated magnetic beads and anti-human IgG-conjugated hollow gold nanospheres (HGNs) were used as substrates and SERS nano-tags, respectively. First, its detection sensitivity was evaluated using anti-CCP standard solutions. Then quantitative anti-CCP levels, determined by the SERS-based assay, were compared with those obtained from three commercially available anti-CCP assay kits (Immunoscan CCPlus, ImmunnLisa™ CCP and BioPlex™ 2200) to assess its potential utility as a clinical tool. Finally, clinical samples from 20 RA patients were investigated using them. In the SERS-based assay, the anti-CCP level in human serum was successfully determined by monitoring the characteristic Raman peak intensity of SERS nano-tags. The diagnostic performance of our SERS-based immunoassay for clinical samples shows a good agreement with those measured by three commercial anti-CCP kits. In addition, our SERS-based assay results are more consistent in the low concentration range (0-25 U/mL) than those achieved by the commercial kits. Accordingly, it is estimated that the SERS-based assay is a potentially useful diagnostic tool for early diagnosis of RA. © 2015 SPIE.
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Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF BIONANO ENGINEERING > 1. Journal Articles
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