Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step
- Authors
- Lee, Ji-Hyeok; Ko, Ju-Young; Oh, Jae-Young; Kim, Chul-Young; Lee, Hee-Ju; Kim, Jaeil; Jeon, You-Jin
- Issue Date
- Sep-2014
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Centrifugal partition chromatography (CPC); Phlorotannin; Ecklonia cava
- Citation
- FOOD CHEMISTRY, v.158, pp.433 - 437
- Indexed
- SCIE
SCOPUS
- Journal Title
- FOOD CHEMISTRY
- Volume
- 158
- Start Page
- 433
- End Page
- 437
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/21951
- DOI
- 10.1016/j.foodchem.2014.02.112
- ISSN
- 0308-8146
- Abstract
- d Various bioactive phlorotannins of Ecklonia cave (e.g., dieckol, eckol, 6,6-bieckol, phloroglucinol, phloroeckol, and phlorofucofuroeckol-A) are reported. However, their isolation and purification are not easy. Centrifugal partition chromatography (CPC) can be used to efficiently purify the various bioactive-compounds efficiently from E. cava. Phlorotannins are successfully isolated from the ethyl acetate (EtOAc) fraction of E. cava by CPC with a two-phase solvent system comprising n-hexane:EtOAc:methanol:water (2:7:3:7, v/v) solution. The dieckol (fraction 1, 40.2 mg), phlorofucofuroeckol-A (fraction III, 31.1 mg), and fraction 11 (34.1 mg) with 2,7-phloroglucino1-6,6-bieckol and pyrogallol-phloroglucino1-6,6-bieckol are isolated from the crude extract (500 mg) by a one-step CPC system. The purities of the isolated dieckol and phlorofucofuroeckol-A are >= 90% according to high performance liquid chromatography (HPLC) and electrospray ionization multi stage tandem mass spectrometry analyses. The purified 2,7-phloroglucino1-6,6-bieckol and pyrogallol-phloroglucino1-6,6-bieckol are collected from fraction II by recycle-HPLC. Thus, the CPC system is useful for easy and simple isolation of phlorotannins from E. cava. (C) 2014 Elsevier Ltd. All rights reserved.
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