Synthesis of 3 '-O-fluorescently mono-modified reversible terminators and their uses in sequencing-by-synthesis
- Authors
- Kim, Da-Rae; Kim, Taek-Soo; Kim, Eunsun; Min, Sun-Joon; Shin, Dongyun; Ahn, Dae-Ro
- Issue Date
- Jan-2014
- Publisher
- Pergamon Press Ltd.
- Keywords
- Sequencing-by-synthesis; Reversible terminator; Modified nucleotide; DNA polymerase; Triphosphate
- Citation
- Bioorganic and Medicinal Chemistry Letters, v.24, no.1, pp 209 - 213
- Pages
- 5
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Bioorganic and Medicinal Chemistry Letters
- Volume
- 24
- Number
- 1
- Start Page
- 209
- End Page
- 213
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/24099
- DOI
- 10.1016/j.bmcl.2013.11.040
- ISSN
- 0960-894X
1464-3405
- Abstract
- Next-generation sequencing (NGS) technologies recently developed are now used for study of genomes from various organisms. Sequencing-by-synthesis (SBS) is a key strategy in the NGS. The SBS uses nucleotides so-called dual-modified reversible terminators (DRTs) in which bases are labeled with fluorophores and 3'-OH is protected with a reversibly cleavable chemical group, respectively. In this study, we examined the possibility of performing SBS with mono-modified reversible terminators (MRTs), in which the reversible blocking group on the 3'-OH plays a dual role as a fluorescent signal report as well as a chemical protection. We studied cyclic reversible termination by using two MRTs (dA and dT), wherein the modifications were two different fluorophores and cleavable to regenerate a free 3'-OH. We here demonstrated that SBS could be achieved with incorporation of MRTs by a DNA polymerase and correct base-calls based on the two different colors from the fluorophores. (C) 2013 Elsevier Ltd. All rights reserved.
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