Preparative isolation of sargachromanol E from Sargassum siliquastrum by centrifugal partition chromatography and its anti-inflammatory activity
DC Field | Value | Language |
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dc.contributor.author | Lee, Ji-Hyeok | - |
dc.contributor.author | Ko, Ju-Young | - |
dc.contributor.author | Samarakoon, Kalpa | - |
dc.contributor.author | Oh, Jae-Young | - |
dc.contributor.author | Heo, Soo-Jin | - |
dc.contributor.author | Kim, Chul-Young | - |
dc.contributor.author | Nah, Jae-Woon | - |
dc.contributor.author | Jang, Mi-Kyeong | - |
dc.contributor.author | Lee, Jung-Suck | - |
dc.contributor.author | Jeon, You-Jin | - |
dc.date.accessioned | 2021-06-23T02:03:29Z | - |
dc.date.available | 2021-06-23T02:03:29Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2013-12 | - |
dc.identifier.issn | 0278-6915 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/26289 | - |
dc.description.abstract | Centrifugal partition chromatography (CPC) can be used to isolate various bioactive compounds from natural materials by one-step. We confirmed antioxidative compounds existed in chloroform (CHCl3) fraction of Sargassum siliquastrum using online-HPLC. Fractions (A, B, C, D and E) were separated from the CHCl3 fraction by preparative CPC (n-hexane:ethyl acetate:methanol:water, 5:5:7:3, v/v). In this study, we proved that the isolated compounds exhibit anti-inflammatory activities using lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. The fraction A which exhibited the strongest inhibitory effect on nitric oxide (NO) production level, was confirmed as sargachromanol E by LC-MS-ESI, H-1 NMR and C-13 NMR data. The sargachromanol E significantly reduced the inflammatory response in LPS induced macrophages, decreasing LPS-induced transcription factor of pro-inflammatory cyclooxygenase-2, NO synthase, phosphate P38, phosphate ERK1/2, LPS-stimulated tumor-necrosis factor alpha, interleukin-1 beta and prostaglandin E2 release. In conclusion, it was suggested that sargachromanol E inhibited inflammation in LPS induced RAW 264.7 cells via MAPK pathway. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Preparative isolation of sargachromanol E from Sargassum siliquastrum by centrifugal partition chromatography and its anti-inflammatory activity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chul-Young | - |
dc.identifier.doi | 10.1016/j.fct.2013.08.010 | - |
dc.identifier.scopusid | 2-s2.0-84883680989 | - |
dc.identifier.wosid | 000329960400009 | - |
dc.identifier.bibliographicCitation | FOOD AND CHEMICAL TOXICOLOGY, v.62, pp.54 - 60 | - |
dc.relation.isPartOf | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.citation.title | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.citation.volume | 62 | - |
dc.citation.startPage | 54 | - |
dc.citation.endPage | 60 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | BROWN-ALGA | - |
dc.subject.keywordPlus | PLASTOQUINONES | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | Centrifugal partition chromatography | - |
dc.subject.keywordAuthor | Sargachromanol E | - |
dc.subject.keywordAuthor | Sargassum siliquastrum | - |
dc.subject.keywordAuthor | Antiinflammatory activity | - |
dc.subject.keywordAuthor | MAPK pathway | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0278691513005528?via%3Dihub | - |
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