Preparative isolation of sargachromanol E from Sargassum siliquastrum by centrifugal partition chromatography and its anti-inflammatory activity
- Authors
- Lee, Ji-Hyeok; Ko, Ju-Young; Samarakoon, Kalpa; Oh, Jae-Young; Heo, Soo-Jin; Kim, Chul-Young; Nah, Jae-Woon; Jang, Mi-Kyeong; Lee, Jung-Suck; Jeon, You-Jin
- Issue Date
- Dec-2013
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Centrifugal partition chromatography; Sargachromanol E; Sargassum siliquastrum; Antiinflammatory activity; MAPK pathway
- Citation
- FOOD AND CHEMICAL TOXICOLOGY, v.62, pp.54 - 60
- Indexed
- SCIE
SCOPUS
- Journal Title
- FOOD AND CHEMICAL TOXICOLOGY
- Volume
- 62
- Start Page
- 54
- End Page
- 60
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/26289
- DOI
- 10.1016/j.fct.2013.08.010
- ISSN
- 0278-6915
- Abstract
- Centrifugal partition chromatography (CPC) can be used to isolate various bioactive compounds from natural materials by one-step. We confirmed antioxidative compounds existed in chloroform (CHCl3) fraction of Sargassum siliquastrum using online-HPLC. Fractions (A, B, C, D and E) were separated from the CHCl3 fraction by preparative CPC (n-hexane:ethyl acetate:methanol:water, 5:5:7:3, v/v). In this study, we proved that the isolated compounds exhibit anti-inflammatory activities using lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. The fraction A which exhibited the strongest inhibitory effect on nitric oxide (NO) production level, was confirmed as sargachromanol E by LC-MS-ESI, H-1 NMR and C-13 NMR data. The sargachromanol E significantly reduced the inflammatory response in LPS induced macrophages, decreasing LPS-induced transcription factor of pro-inflammatory cyclooxygenase-2, NO synthase, phosphate P38, phosphate ERK1/2, LPS-stimulated tumor-necrosis factor alpha, interleukin-1 beta and prostaglandin E2 release. In conclusion, it was suggested that sargachromanol E inhibited inflammation in LPS induced RAW 264.7 cells via MAPK pathway. (C) 2013 Elsevier Ltd. All rights reserved.
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