Different GATA Factors Dictate CCR3 Transcription in Allergic Inflammatory Cells in a Cell Type-Specific Manner
- Authors
- Kong, Su-Kang; Kim, Byung Soo; Uhm, Tae Gi; Lee, Wonyong; Lee, Gap Ryol; Park, Choon-Sik; Lee, Chul-Hoon; Chung, Ii Yup
- Issue Date
- Jun-2013
- Publisher
- American Association of Immunologists
- Keywords
- AIRWAY EPITHELIAL-CELLS; HEMATOPOIETIC-CELLS; EOSINOPHIL LINEAGE; GENE-EXPRESSION; EOTAXIN RECEPTOR; CHEMOKINE RECEPTOR CCR3; HUMAN MAST-CELLS; MAJOR BASIC-PROTEIN; T-CELLS; CHROMATIN OCCUPANCY
- Citation
- Journal of Immunology, v.190, no.11, pp.5747 - 5756
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Immunology
- Volume
- 190
- Number
- 11
- Start Page
- 5747
- End Page
- 5756
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/27618
- DOI
- 10.4049/jimmunol.1203542
- ISSN
- 0022-1767
- Abstract
- The chemokine receptor CCR3 is expressed in prominent allergic inflammatory cells, including eosinophils, mast cells, and Th2 cells. We previously identified a functional GATA element within exon 1 of the CCR3 gene that is responsible for GATA-1-mediated CCR3 transcription. Because allergic inflammatory cells exhibit distinct expression patterns of different GATA factors, we investigated whether different GATA factors dictate CCR3 transcription in a cell type specific manner. GATA-2 was expressed in EoL-1 eosinophilic cells, GATA-1 and GATA-2 were expressed in HMC-1 mast cells, and GATA-3 was preferentially expressed in Jurkat cells. Unlike a wild-type CCR3 reporter, reporters lacking the functional GATA element were not active in any of the three cell types, implying the involvement of different GATA factors in CCR3 transcription. RNA interference assays showed that small interfering RNAs specific for different GATA factors reduced CCR3 reporter activity in a cell type-specific fashion. Consistent with these findings, chromatin immunoprecipitation and EMSA analyses demonstrated cell type-specific binding of GATA factors to the functional GATA site. More importantly, specific inhibition of the CCR3 reporter activity by different GATA small interfering RNAs was well preserved in respective cell types differentiated from cord blood; in particular, GATA-3 was entirely responsible for reporter activity in Th2 cells and replaced the role predominantly played by GATA-1 and GATA-2. These results highlight a mechanistic role of GATA factors in which cell type specific expression is the primary determinant of transcription of the CCR3 gene in major allergic inflammatory cells.
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- COLLEGE OF PHARMACY > DEPARTMENT OF PHARMACY > 1. Journal Articles
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