Non-ionic detergents Nonidet P-40 and Triton X-100 increase enzymatic activity of plasmin
DC Field | Value | Language |
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dc.contributor.author | Trinh, Trang H. T. | - |
dc.contributor.author | Kim, Jeeyoung | - |
dc.contributor.author | Lee, Chul-Hoon | - |
dc.contributor.author | Ryou, Chongsuk | - |
dc.date.accessioned | 2021-06-22T10:03:29Z | - |
dc.date.available | 2021-06-22T10:03:29Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2019-04 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/3007 | - |
dc.description.abstract | Plasmin is a potent serin protease involved in a variety of biological functions, such as fibrinolysis and tissue remodeling. On performing an in vitro control assay to measure the activity of endogenous plasmin in cell lysates, a stimulatory effect of non-ionic detergent NP-40 on plasmin activity was discovered. Another non-ionic detergent, TX-100, also enhanced plasmin activity, while ionic detergents sodium deoxycholate and sodiem dodecyl sulfate abolished plasmin enzyme activity. Kinetic analysis of plasmin activity in the presence of NP-40 and TX-100 demonstrated an increase in V-max; however, there was no change in K-m values, suggesting that these detergents stimulate plasmin activity in a non-competitive manner. Fibrin plate assay indicates that NP-40 and TX-100 functionally stimulate plasmin activity by showing a dose-dependent increase in fibrinolysis. (C) 2019 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Academic Press | - |
dc.title | Non-ionic detergents Nonidet P-40 and Triton X-100 increase enzymatic activity of plasmin | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Chul-Hoon | - |
dc.contributor.affiliatedAuthor | Ryou, Chongsuk | - |
dc.identifier.doi | 10.1016/j.bbrc.2019.03.052 | - |
dc.identifier.scopusid | 2-s2.0-85062893727 | - |
dc.identifier.wosid | 000468254400028 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, v.512, no.2, pp.314 - 318 | - |
dc.relation.isPartOf | Biochemical and Biophysical Research Communications | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.volume | 512 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 314 | - |
dc.citation.endPage | 318 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | FIBRINOLYSIS | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | DISORDER | - |
dc.subject.keywordPlus | SALTS | - |
dc.subject.keywordAuthor | Non-ionic | - |
dc.subject.keywordAuthor | Detergent | - |
dc.subject.keywordAuthor | Enzyme | - |
dc.subject.keywordAuthor | Plasmin | - |
dc.subject.keywordAuthor | Non-competitive | - |
dc.subject.keywordAuthor | Stimulator | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0006291X19304292?via%3Dihub | - |
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