Non-ionic detergents Nonidet P-40 and Triton X-100 increase enzymatic activity of plasmin
- Authors
- Trinh, Trang H. T.; Kim, Jeeyoung; Lee, Chul-Hoon; Ryou, Chongsuk
- Issue Date
- Apr-2019
- Publisher
- Academic Press
- Keywords
- Non-ionic; Detergent; Enzyme; Plasmin; Non-competitive; Stimulator
- Citation
- Biochemical and Biophysical Research Communications, v.512, no.2, pp.314 - 318
- Indexed
- SCIE
SCOPUS
- Journal Title
- Biochemical and Biophysical Research Communications
- Volume
- 512
- Number
- 2
- Start Page
- 314
- End Page
- 318
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/3007
- DOI
- 10.1016/j.bbrc.2019.03.052
- ISSN
- 0006-291X
- Abstract
- Plasmin is a potent serin protease involved in a variety of biological functions, such as fibrinolysis and tissue remodeling. On performing an in vitro control assay to measure the activity of endogenous plasmin in cell lysates, a stimulatory effect of non-ionic detergent NP-40 on plasmin activity was discovered. Another non-ionic detergent, TX-100, also enhanced plasmin activity, while ionic detergents sodium deoxycholate and sodiem dodecyl sulfate abolished plasmin enzyme activity. Kinetic analysis of plasmin activity in the presence of NP-40 and TX-100 demonstrated an increase in V-max; however, there was no change in K-m values, suggesting that these detergents stimulate plasmin activity in a non-competitive manner. Fibrin plate assay indicates that NP-40 and TX-100 functionally stimulate plasmin activity by showing a dose-dependent increase in fibrinolysis. (C) 2019 Elsevier Inc. All rights reserved.
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