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Non-ionic detergents Nonidet P-40 and Triton X-100 increase enzymatic activity of plasmin

Authors
Trinh, Trang H. T.Kim, JeeyoungLee, Chul-HoonRyou, Chongsuk
Issue Date
Apr-2019
Publisher
Academic Press
Keywords
Non-ionic; Detergent; Enzyme; Plasmin; Non-competitive; Stimulator
Citation
Biochemical and Biophysical Research Communications, v.512, no.2, pp.314 - 318
Indexed
SCIE
SCOPUS
Journal Title
Biochemical and Biophysical Research Communications
Volume
512
Number
2
Start Page
314
End Page
318
URI
https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/3007
DOI
10.1016/j.bbrc.2019.03.052
ISSN
0006-291X
Abstract
Plasmin is a potent serin protease involved in a variety of biological functions, such as fibrinolysis and tissue remodeling. On performing an in vitro control assay to measure the activity of endogenous plasmin in cell lysates, a stimulatory effect of non-ionic detergent NP-40 on plasmin activity was discovered. Another non-ionic detergent, TX-100, also enhanced plasmin activity, while ionic detergents sodium deoxycholate and sodiem dodecyl sulfate abolished plasmin enzyme activity. Kinetic analysis of plasmin activity in the presence of NP-40 and TX-100 demonstrated an increase in V-max; however, there was no change in K-m values, suggesting that these detergents stimulate plasmin activity in a non-competitive manner. Fibrin plate assay indicates that NP-40 and TX-100 functionally stimulate plasmin activity by showing a dose-dependent increase in fibrinolysis. (C) 2019 Elsevier Inc. All rights reserved.
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