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Monoallelic gene targeting in hypoblast stem cells reveals X chromosome inactivation
- Authors
- Han, Dongjun; Binas, Bert
- Issue Date
- Oct-2012
- Publisher
- Academic Press
- Keywords
- Stem cells; Hypoblast stem cells; Homologous recombination; Gene targeting; Hypoxanthine phosphoribosyl transferase; X chromosome inactivation
- Citation
- Biochemical and Biophysical Research Communications, v.427, no.3, pp 563 - 567
- Pages
- 5
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Biochemical and Biophysical Research Communications
- Volume
- 427
- Number
- 3
- Start Page
- 563
- End Page
- 567
- ISSN
- 0006-291X
1090-2104
- Abstract
- We recently isolated hypoblast stem cells (HypoSC), which are related to embryonic stem (ES) cells. ES cells efficiently perform homologous recombination (HR) and lack X chromosome inactivation (Xi), but it is unknown whether the same applies to HypoSC. Using the X-linked hypoxanthine phosphoribosyl transferase (HPRT) gene, we find that HypoSC perform HR with similar frequency as ES cells. Monoallelic targeting in female HypoSC eliminated HPRT gene expression, implying epigenetic inactivation of the other allele. Although density-induced differentiation complicated selection, the targeted clones maintained their original properties. These results will facilitate targeted genetic manipulation of HypoSC and the study of Xi. (C) 2012 Elsevier Inc. All rights reserved.
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