Proteomics-based identification and characterization of biotype-specific carboxylesterase 2 putatively associated with insecticide resistance in Bemisia tabaci
- Authors
- Kang, Soyoung; Lee, Hye Jung; Kim, Young Ho; Kwon, Deok Ho; Oh, Jung Hun; Kim, Bum Jun; Lim, Kook Jin; Lee, Seunghwan; Hwang, Seung Yong; Lee, Si Hyeock
- Issue Date
- Sep-2012
- Publisher
- 한국응용곤충학회
- Keywords
- Bemisia tabaci; Biotypes; Proteomic analysis; Carboxylesterase; Overexpression
- Citation
- Journal of Asia-Pacific Entomology, v.15, no.3, pp.389 - 396
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Journal of Asia-Pacific Entomology
- Volume
- 15
- Number
- 3
- Start Page
- 389
- End Page
- 396
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/32164
- DOI
- 10.1016/j.aspen.2012.03.002
- ISSN
- 1226-8615
- Abstract
- Proteomic differences between Bemisia tabaci biotypes (B and Q) were investigated by two-dimensional gel electrophoresis in conjunction with mass spectroscopic analysis. Among several protein spots specific to biotype B, carboxylesterase 2 (Coe2) was significantly more expressed in biotype B. Phylogenetic analysis demonstrated the close relationship of Coe2 with Myzus persicae esterase E4. Comparison of full-length cDNA sequences of Coe2 revealed no amino acid differences in functionally important conserved regions between biotypes B and Q. The transcription level of the Coe2 gene (coe2) was 5.8-fold higher in biotype B than in biotype Q but the coe2 copy number was not different between biotypes, suggesting that the overexpression of Coe2 was due to transcriptional up-regulation. Native isoelectric focusing followed by mass spectrophotometric analysis confirmed that the overexpressed pl 5.7 esterase in biotype B was Coe2. In-gel inhibition of Coe2 by three insecticides indicated the interaction of Coe2 with chlorpyrifos-methyl oxon and permethrin, but not with imidacloprid. These findings suggest that overexpression of Coe2 in biotype B can confer chemical defense against pyrethroid and organophosphate insecticides, perhaps by sequestration and hydrolysis, as seen in M. persicae E4. Finally, utility of Coe2 as a potential biotype-specific protein marker is discussed. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2012. Published by Elsevier B.V. All rights reserved.
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