High-Performance Liquid Chromatography-Based Multivariate Analysis to Predict the Estrogenic Activity of an Epimedium koreanum Extract
- Authors
- Islam, M. Nurul; Kim, Unyong; Kim, Dong-Hyun; Dong, Mi-Sook; Yoo, Hye Hyun
- Issue Date
- May-2012
- Publisher
- Japan Society for Bioscience Biotechnology and Agrochemistry/Nippon Nogeikagaku Kai
- Keywords
- Epimedium koreanum Nakai; high-performance liquid chromatography (HPLC); multi-variate analysis; estrogenic activity
- Citation
- Bioscience, Biotechnology and Biochemistry, v.76, no.5, pp.923 - 927
- Indexed
- SCIE
SCOPUS
- Journal Title
- Bioscience, Biotechnology and Biochemistry
- Volume
- 76
- Number
- 5
- Start Page
- 923
- End Page
- 927
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/33058
- DOI
- 10.1271/bbb.110922
- ISSN
- 0916-8451
- Abstract
- This study characterizes the correlation between the chemical fingerprint and estrogenic activity of an Epimedium koreanum extract. The estrogenic activity of 31 E. koreanum extract samples was evaluated by a luciferase reporter gene assay, and the samples were classified into 3 groups based on their bioactivity. A chemical fingerprint analysis was performed on each sample by high-performance liquid chromatography (HPLC), and 44 common peaks were selected from the chromatogram and used as a dataset for a pattern recognition analysis. A canonical discriminant analysis performed on this dataset determined a distinct distribution of the samples according to their estrogenic activity on the scoring plot. The classification results showed that 90.3% of the original grouped cases had been correctly classified. The total content of the 4 major extract compounds, epimedin A, epimedin B, epimedin C, and icariin, exhibited good correlation (r = 0.784) with the estrogenic activities of the respective extracts. This chromatographic fingerprint-chemometric analysis system could be useful for predicting the E. koreanum pharmacological activity and consequent biological activity-relevant quality control assessment.
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