Bioethanol production from micro-algae, Schizocytrium sp., using hydrothermal treatment and biological conversion
- Authors
- Kim, Jung Kon; Um, Byung-Hwan; Kim, Tae Hyun
- Issue Date
- Feb-2012
- Publisher
- KOREAN INST CHEM ENGINEERS+
- Keywords
- Biofuel; Simultaneous Saccharification and Fermentation (SSF); Fractionation; KO11; Hot Water; Schizochytrium sp.
- Citation
- KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.29, no.2, pp.209 - 214
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- KOREAN JOURNAL OF CHEMICAL ENGINEERING
- Volume
- 29
- Number
- 2
- Start Page
- 209
- End Page
- 214
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/33863
- DOI
- 10.1007/s11814-011-0169-3
- ISSN
- 0256-1115
- Abstract
- Hydrothermal fractionation for micro-algae, Schizocytrium sp., was investigated to separate sugars, lipids, and proteins. This fractionation process produced protein-rich solid cake and liquid hydrolysates, which contained oligomeric sugars and lipids. Oligomeric sugars and lipids were easily separated by liquid-liquid separation. Sugars in the separated hydrolyzate were determined to be mainly D-glucose and L-galactose. Fractionation conditions were optimized by response surface methodology (RSM). Optimal conditions were found to be 115.5 degrees C of reaction temperature, 46.7 min of reaction time, and 25% (w/w) of solid loading. The model predicted that maximum oligomeric sugar yield (based on untreated micro-algae weight), which can be recovered by hydrothermal fractionation at the optimum conditions, was 19.4 wt% (based on the total biomass weight). Experimental results were in agreement with the model prediction of 16.6 wt%. Production of bioethanol using micro-algae-induced glucan and E. coli KO11 was tested with SSF (simultaneous saccharification and fermentation), which resulted in 11.8 g-ethanol/l was produced from 25.7 g/l of glucose; i.e. the theoretical maximum ethanol yield based on glucan in hydrolyzate was 89.8%.
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