HY251, a Novel Decahydrocyclopenta[a]indene Analog, Induces Apoptosis via tBid-Mediated Intrinsic Pathway in Human Ovarian Cancer PA-1 Cells
- Authors
- Suh, Hyewon; Ko-woon Choi; Myung Sic Kim; Jeong-Hyeon Kim; Sun-Young Noh; Sung, Moon-Hee; Lee, Chul-Hoon
- Issue Date
- Nov-2012
- Publisher
- 한국미생물·생명공학회
- Keywords
- Aralia continentalis; apoptotic induction; tBid; cytochrome c; PA-1 cells
- Citation
- Journal of Microbiology and Biotechnology, v.22, no.11, pp 1591 - 1595
- Pages
- 5
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 22
- Number
- 11
- Start Page
- 1591
- End Page
- 1595
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/34350
- DOI
- 10.4014/jmb.1209.09022
- ISSN
- 1017-7825
1738-8872
- Abstract
- We previously isolated a novel compound, HY251, with the molecular structure of 3-propyl-2-vinyl-1,2,3,3a,3b,6,7,7a,8,8adecahydrocyclopenta[a]indene-3,3a,7a,8a-tetraol from the roots of Aralia continentalis. The current study was designed to evaluate the detailed molecular mechanisms underlying the apoptotic induction by HY251 in human ovarian cancer PA-1 cells. TUNEL assay and Western blot analyses revealed an appreciable apoptotic induction in PA-1 cells treated with 60 μM of HY251 for 24 h. This apoptotic induction was associated with caspase-8-dependent Bid cleavage, which in turn resulted in the formation of pro-apoptotic truncated Bid (tBid), and activation of caspase-9 and -3, as well as the cleavage of poly(ADPribose)polymerase (PARP). Moreover, we found that this death event was also associated with the significant upregulation and activation of the p53 tumor-suppressor protein through phosphorylation at Ser15. Therefore, we suggest that HY251 may be a potent cancer chemotherapeutic candidate for the treatment of ovarian cancer.
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