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PREPARATIVE ISOLATION AND PURIFICATION OF FLAVONOIDS FROM PTEROCARPUS SALTALINUS USING CENTRIFUGAL PARTITION CHROMATOGRAPHY
- Issue Date
- Oct-2012
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- quercetin; centrifugal partition chromatography; dihydrokaempferol; eryodictyol; naringenin; Pterocarpus santalinus; taxifolin
- Citation
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.35, no.17, pp 2462 - 2470
- Pages
- 9
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
- Volume
- 35
- Number
- 17
- Start Page
- 2462
- End Page
- 2470
- ISSN
- 1082-6076
1520-572X
- Abstract
- A centrifugal partition chromatography (CPC) was applied to separate and purify flavonoids from the extract of Pterocarpus santalinus. Three flavonoids, taxifolin (21 mg), dihydrokaempferol (18 mg), and naringenin (16 mg) were successfully separated from 500 mg of ethyl acetate fraction of P. santalinus using centrifugal partition chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (4:5: 4:5, v/v) in single CPC run. Eryodictyol (8 mg) and quercetin (5 mg) were further purified by HPLC. The purities of the isolated compounds were determined to be over 90% by HPLC analysis and their structures were elucidated by H-1 NMR, C-13 NMR, and ESI-MS.
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Related Researcher
- Kim, Chul Young
- COLLEGE OF PHARMACY (DEPARTMENT OF PHARMACY)