PREPARATIVE ISOLATION AND PURIFICATION OF PRENYLATED ISOFLAVONOIDS FROM CUDRANIA TRICUSPIDATA FRUITS USING CENTRIFUGAL PARTITION CHROMATOGRAPHY
- Authors
- Jeon, Je-Seung; Kim, Sang-Min; Lee, Hee Ju; Um, Byung Hun; Kim, Hak Ki; Kim, Chul Young
- Issue Date
- Jun-2012
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- 4 ' -O-methylalpinumisoflavone; 6,8-diprenylgenistein; 6,8-diprenylorobol; centrifugal partition chromatography; Cudrania tricuspidata; prenylated isoflavonoids
- Citation
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.35, no.11, pp.1607 - 1615
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
- Volume
- 35
- Number
- 11
- Start Page
- 1607
- End Page
- 1615
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/36289
- DOI
- 10.1080/10826076.2011.621151
- ISSN
- 1082-6076
- Abstract
- Prenylated isoflavonoids such as 6,8-diprenylorobol, 6,8-diprenylgenistein and 4'-O-methylalpinumisoflavone were purified from n-hexane extract of C. tricuspidata fruits using centrifugal partition chromatography (CPC) with a n-hexane-ethyl acetate-methanol-water (7: 3: 6: 4, v/v, ascending mode) solvent system in a one-step within 200 min. One-step preparative CPC yielded 22.4 mg of 6,8-diprenylorobol and 26.7 mg of 6,8-diprenylgenistein with 95% and 85% purity, respectively, from 350 mg of crude hexane extract. Further purification by crystallization in methanol yielded 4'-O-methylalpinumisoflavone (9.3 mg) at over 95% purity based on the HPLC analysis. The chemical structures of purified compounds were elucidated by H-1, C-13 NMR, and ESI-MS.
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