A simple isocratic HPLC method for the simultaneous determination of bioactive components of Scutellariae Radix extract
- Authors
- Islam, M. Nurul; Chung, Hye Jin; Kim, Dong-Hyun; Yoo, Hye Hyun
- Issue Date
- Nov-2012
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Scutellariae Radix; HPLC; isocratic elution; quality control
- Citation
- NATURAL PRODUCT RESEARCH, v.26, no.21, pp.1957 - 1962
- Indexed
- SCIE
SCOPUS
- Journal Title
- NATURAL PRODUCT RESEARCH
- Volume
- 26
- Number
- 21
- Start Page
- 1957
- End Page
- 1962
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/36294
- DOI
- 10.1080/14786419.2011.631134
- ISSN
- 1478-6419
- Abstract
- Scutellariae Radix, the dried root of Scutellaria baicalensis Georgi, has been widely used in Asian countries for the treatment of dermatitis, diarrhoea, inflammatory disease and hepatic disease. A simple, sensitive and precise reversed-phase liquid chromatographic method with isocratic elution was developed to simultaneously determine four bioactive compounds in Scutellariae Radix: baicalein, baicalin, wogonin and wogonoside. Chromatographic analysis was performed on a YMC Pack Pro C-8 column (150 x 4.6mm(2), 3 mu m), with a mobile phase of 0.1% formic acid : acetonitrile (70 : 30, v/v) at a flow rate of 1.0mLmin(-1), and UV detection at 280 nm. Linear behaviour was observed over the investigated concentration range (0.25-10 mu gmL(-1)) for all analytes, with a correlation coefficient of >0.997. The intra-and inter-day precisions were <8.07%, and accuracies were 92.3-102.9%. This method was successfully applied for the analysis of marker compounds for the quality control of Scutellariae Radix extract.
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