Cell and Nuclear Enlargement of SW480 Cells Induced by a Plant Lignan, Arctigenin: Evaluation of Cellular DNA Content Using Fluorescence Microscopy and Flow Cytometryopen access
- Authors
- Kang, Kyungsu; Lee, Hee Ju; Yoo, Ji-Hye; Jho, Eun Hye; Kim, Chul Young; Kim, Minkyun; Nho, Chu Won
- Issue Date
- Aug-2011
- Publisher
- MARY ANN LIEBERT INC
- Keywords
- Humans; Lignans; drug effect; DNA content; cell size; cell DNA; nuclear size; Flow Cytometry; Cell Division; parameter; G2 Phase; flow cytometry; priority journal; cancer cell culture; human; cell enlargement; Saussurea; Antineoplastic Agents; Cell Size
- Citation
- DNA AND CELL BIOLOGY, v.30, no.8, pp.623 - 629
- Indexed
- SCIE
SCOPUS
- Journal Title
- DNA AND CELL BIOLOGY
- Volume
- 30
- Number
- 8
- Start Page
- 623
- End Page
- 629
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/37261
- DOI
- 10.1089/dna.2010.1199
- ISSN
- 1044-5498
- Abstract
- Arctigenin is a natural plant lignan previously shown to induce G(2)/M arrest in SW480 human colon cancer cells as well as AGS human gastric cancer cells, suggesting its use as a possible cancer chemopreventive agent. Changes in cell and nuclear size often correlate with the functionality of cancer-treating agents. Here, we report that arctigenin induces cell and nuclear enlargement of SW480 cells. Arctigenin clearly induced the formation of giant nuclear shapes in SW480, as demonstrated by fluorescence microscopic observation and quantitative determination of nuclear size. Cell and nuclear size were further assessed by flow cytometric analysis of light scattering and fluorescence pulse width after propidium iodide staining. FSC-H and FL2-W values (parameters referring to cell and nuclear size, respectively) significantly increased after arctigenin treatment; the mean values of FSC-H and FL2-W in arctigenin-treated SW480 cells were 572.6 and 275.1, respectively, whereas those of control cells were 482.0 and 220.7, respectively. Our approach may provide insights into the mechanism behind phytochemical-induced cell and nuclear enlargement as well as functional studies on cancer-treating agents.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF PHARMACY > DEPARTMENT OF PHARMACY > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/37261)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.