An Integrated Microfluidic Device for Rapid Cell Lysis and DNA Purification of Epithelial Cell Samples
- Authors
- Ha, Seung-Mo; Cho, Woong; Ahn, Yoomin; Hwang, Seung Yong
- Issue Date
- May-2011
- Publisher
- American Scientific Publishers
- Keywords
- Microfluidic Device; Cell Lysis; DNA Purification
- Citation
- Journal of Nanoscience and Nanotechnology, v.11, no.5, pp.4250 - 4253
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Nanoscience and Nanotechnology
- Volume
- 11
- Number
- 5
- Start Page
- 4250
- End Page
- 4253
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/38119
- DOI
- 10.1166/jnn.2011.3640
- ISSN
- 1533-4880
- Abstract
- In this paper, we describe the design and fabrication of a microfluidic device for cell lysis and DNA purification, and the results of device tests using a real sample of buccal cells. Cell lysis was thermally executed for two minutes at 80 degrees C in a serpentine type microreactor (20 mu L) using an Au microheater with a microsensor. The DNA was then mixed with other residual products and purified by a new filtration process involving micropillars and 50-80 mu m microbeads. The entire process of sample loading, cell lysis, DNA purification, and sample extraction was successfully completed in the microchip within five minutes. Sample preparation within the microchip was verified by performing a SY158 gene PCR analysis and gel electrophoresis on the products obtained from the chip. The new purification method enhanced DNA purity from 0.93 to 1.62 after purification.
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Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > ERICA 의약생명과학과 > 1. Journal Articles
- COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF MECHANICAL ENGINEERING > 1. Journal Articles
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