Hydrophillic Interaction Chromatography-tandem Mass Spectrometry Method for Identification and Quantitation of 5-MeO-DIPT and its Metabolites in Rat Urine
- Authors
- Kim, Yoon; Kim, Un Yong; In, Moon Kyo; Lee, Jaeick; Kwon, Oh-Seung; Yoo, Hye Hyun
- Issue Date
- Apr-2011
- Publisher
- 대한화학회
- Keywords
- 5-MeO-DIPT; HILIC-MS/MS; Metabolites; Profiling; Quantitation
- Citation
- Bulletin of the Korean Chemical Society, v.32, no.4, pp.1158 - 1164
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Bulletin of the Korean Chemical Society
- Volume
- 32
- Number
- 4
- Start Page
- 1158
- End Page
- 1164
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/38130
- DOI
- 10.5012/bkcs.2011.32.4.1158
- ISSN
- 0253-2964
- Abstract
- 5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), a psychoactive tryptamine derivative, is a hallucinogenic drug of abuse. In this study, 5-OH-DIPT and its metabolites were identified and the quantitative method was developed and validated by using hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS). Chromatographic separation was achieved on an Atlantis HILIC silica column (5 mu m, 100 x 2.1 mm). The metabolites of 5-MeO-DIPT in rat urine were characterized via Q1 scanning and product ion scanning. As a consequence, 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT and their glucuronide conjugates were detected and identified as the metabolites of 5-MeO-DIPT. Subsequently, a quantitative method for 5-MeO-DIPT and its major metabolites, 5-MeO-IPT and 5-OH-DIPT, was developed in multiple reactions monitoring (MRM) mode. The calibration curves for all analytes evidenced good linearity over the concentration range of 11000 ng/mL with linear correlation co-efficients (r(2)) in excess of 0.99. The intra- and inter-day accuracy and precision were 92.2-110.2% and 1.5-9.9%, respectively.
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