Antiplatelet effects of Spatholobus suberectus via inhibition of the glycoprotein IIb/IIIa receptor
- Authors
- Lee, Beom-Joon; Jo, In-Young; Bu, Youngmin; Park, Jae-Woo; Maeng, Sungho; Kang, Hee; Jang, Woochang; Hwang, Deok-Sang; Lee, Wookyoung; Min, Kyoungyoon; Kim, Jong-In; Yoo, Hye Hyun; Lew, Jae-Hwan
- Issue Date
- Mar-2011
- Publisher
- Elsevier BV
- Keywords
- Spatholobus suberectus; Platelet aggregation; Antiplatelet agent; Glycoprotein IIb/IIIa; Thromboxane A(2)
- Citation
- Journal of Ethnopharmacology, v.134, no.2, pp.460 - 467
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Ethnopharmacology
- Volume
- 134
- Number
- 2
- Start Page
- 460
- End Page
- 467
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/38199
- DOI
- 10.1016/j.jep.2010.12.039
- ISSN
- 0378-8741
- Abstract
- Ethnopharmacological relevance: The vine stem of Spatholobus suberectus is a widely used blood-activating and stasis-dispelling medicine for the treatment of diseases related to blood stasis syndrome in traditional medicine in Korea, Japan, and China. Aim of the study: To demonstrate the clinical effects of Spatholobus suberectus against blood stasis syndromes using in vitro and in vivo platelet aggregation studies and to investigate its exact mechanisms. Materials and methods: We extracted vine stems of Spatholobus suberectus, using 95% EtOH (SSE) and investigated its antiplatelet activity on platelet aggregation induced by collagen and ADP in human platelet-rich plasma (PRP). For the mechanism study, a glycoprotein IIb/IIIa (GP IIb/IIIa) assay using flow cytometric analysis and a thromboxane A(2) (TXA(2)) assay were performed. In addition, we investigated the effects of SSE in a thromboembolic mouse model. Results: SSE significantly inhibited ADP- and collagen-induced platelet aggregation in human PRP concentration-dependently without affecting plasma clotting time. It also significantly inhibited fibrinogen binding to the GP IIb/IIIa receptor and partly inhibited the formation of TXA(2). In the in vivo study, oral administration of SSE dose-dependently suppressed the death of thromboembolism model mice induced by intravenous injection of collagen plus epinephrine. Conclusions: SSE showed antiplatelet activity without anticoagulant effects mainly through the inhibition of fibrinogen binding to the GP IIb/IIIa receptor. Our current results support the clinical usage of SSE in the East Asian region treating atherothrombotic diseases and may represent a new natural source to develop antiplatelet agents. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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