Intranasal immunization with plasmid DNA encoding spike protein of SARS-coronavirus/polyethylenimine nanoparticles elicits antigen-specific humoral and cellular immune responsesopen access
- Authors
- Shim, Byoung-Shik; Park, Sung-Moo; Quan, Ji-Shan; Jere, Dhananjay; Chu, Hyuk; Song, Man Ki; Kim, Dong Wook; Jang, Yong-Suk; Yang, Moon-Sik; Han, Seung Hyun; Park, Yong-Ho; Cho, Chong-Su; Yun, Cheol-Heui
- Issue Date
- Dec-2010
- Publisher
- BMC
- Keywords
- cytology; Cell Proliferation; Dendritic Cells; Viral Envelope Proteins; in vitro study; virus antibody; B7 antigen; Immunity; intranasal drug administration; mouse; SARS coronavirus; Immunity, Humoral; interleukin 2; Cell Differentiation; Membrane Glycopr
- Citation
- BMC IMMUNOLOGY, v.11, pp.1 - 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- BMC IMMUNOLOGY
- Volume
- 11
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39252
- DOI
- 10.1186/1471-2172-11-65
- ISSN
- 1471-2172
- Abstract
- Background: Immunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and to prevent the infection caused by SARS-CoV. Polyethylenimine 25K (PEI) is a cationic polymer which effectively delivers the plasmid DNA. Results: In the present study, the immune responses of BALB/c mice immunized via intranasal (i.n.) route with SARS DNA vaccine (pci-S) in a PEI/pci-S complex form have been examined. The size of the PEI/pci-S nanoparticles appeared to be around 194.7 +/- 99.3 nm, and the expression of the S mRNA and protein was confirmed in vitro. The mice immunized with i.n. PEI/pci-S nanoparticles produced significantly (P < 0.05) higher S-specific IgG1 in the sera and mucosal secretory IgA in the lung wash than those in mice treated with pci-S alone. Compared to those in mice challenged with pci-S alone, the number of B220(+) cells found in PEI/pci-S vaccinated mice was elevated. Co-stimulatory molecules (CD80 and CD86) and class II major histocompatibility complex molecules (I-A(d)) were increased on CD11c(+) dendritic cells in cervical lymph node from the mice after PEI/pci-S vaccination. The percentage of IFN-gamma-, TNF-alpha- and IL-2-producing cells were higher in PEI/pci-S vaccinated mice than in control mice. Conclusion: These results showed that intranasal immunization with PEI/pci-S nanoparticles induce antigen specific humoral and cellular immune responses.
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