A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences
DC Field | Value | Language |
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dc.contributor.author | Chung, In-Hyuk | - |
dc.contributor.author | Yoo, Hye Sook | - |
dc.contributor.author | Eah, Jae-Yong | - |
dc.contributor.author | Yoon, Hyun-Kyu | - |
dc.contributor.author | Jung, Jin-Wook | - |
dc.contributor.author | Hwang, Seung Yong | - |
dc.contributor.author | Kim, Chang-Bae | - |
dc.date.accessioned | 2021-06-23T12:40:52Z | - |
dc.date.available | 2021-06-23T12:40:52Z | - |
dc.date.issued | 2010-10 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39524 | - |
dc.description.abstract | DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridization, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국분자세포생물학회 | - |
dc.title | A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.1007/s10059-010-0118-8 | - |
dc.identifier.scopusid | 2-s2.0-78650516319 | - |
dc.identifier.wosid | 000284700000002 | - |
dc.identifier.bibliographicCitation | Molecules and Cells, v.30, no.4, pp 295 - 301 | - |
dc.citation.title | Molecules and Cells | - |
dc.citation.volume | 30 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 295 | - |
dc.citation.endPage | 301 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001493928 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | OLIGONUCLEOTIDE MICROARRAY | - |
dc.subject.keywordPlus | BARCODES | - |
dc.subject.keywordPlus | POSITION | - |
dc.subject.keywordPlus | PROBES | - |
dc.subject.keywordAuthor | birds | - |
dc.subject.keywordAuthor | cytochrome c oxidase I | - |
dc.subject.keywordAuthor | DNA barcoding | - |
dc.subject.keywordAuthor | Korea | - |
dc.subject.keywordAuthor | microarray | - |
dc.subject.keywordAuthor | molecular probes | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s10059-010-0118-8 | - |
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