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A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences

Authors
Chung, In-HyukYoo, Hye SookEah, Jae-YongYoon, Hyun-KyuJung, Jin-WookHwang, Seung YongKim, Chang-Bae
Issue Date
Oct-2010
Publisher
한국분자세포생물학회
Keywords
birds; cytochrome c oxidase I; DNA barcoding; Korea; microarray; molecular probes
Citation
Molecules and Cells, v.30, no.4, pp 295 - 301
Pages
7
Indexed
SCI
SCIE
SCOPUS
KCI
Journal Title
Molecules and Cells
Volume
30
Number
4
Start Page
295
End Page
301
URI
https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39524
DOI
10.1007/s10059-010-0118-8
ISSN
1016-8478
0219-1032
Abstract
DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridization, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.
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ERICA 과학기술융합대학 (ERICA 의약생명과학과)
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