A DNA Microarray for Identification of Selected Korean Birds Based on Mitochondrial Cytochrome c Oxidase I Gene Sequences
- Authors
- Chung, In-Hyuk; Yoo, Hye Sook; Eah, Jae-Yong; Yoon, Hyun-Kyu; Jung, Jin-Wook; Hwang, Seung Yong; Kim, Chang-Bae
- Issue Date
- Oct-2010
- Publisher
- 한국분자세포생물학회
- Keywords
- birds; cytochrome c oxidase I; DNA barcoding; Korea; microarray; molecular probes
- Citation
- Molecules and Cells, v.30, no.4, pp 295 - 301
- Pages
- 7
- Indexed
- SCI
SCIE
SCOPUS
KCI
- Journal Title
- Molecules and Cells
- Volume
- 30
- Number
- 4
- Start Page
- 295
- End Page
- 301
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39524
- DOI
- 10.1007/s10059-010-0118-8
- ISSN
- 1016-8478
0219-1032
- Abstract
- DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridization, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.
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