A DNA microarray for species identification of cetacean animals in Korean water
- Authors
- Park, Jung Youn; Kim, Ji-Hoon; An, Yong-Rock; Kim, Mi-Jung; Lee, Won Sun; An, Cheul Min; Jung, Jin-Wook; Kang, Jung-Ha; Moon, Hyo-Bang; Hwang, Seung Yong
- Issue Date
- Sep-2010
- Publisher
- 한국바이오칩학회
- Keywords
- Cetacean animals; Whale; Species identification; DNA microarray system; Cytochrome c oxidase subunit I
- Citation
- BioChip Journal, v.4, no.3, pp.197 - 203
- Indexed
- SCIE
SCOPUS
KCI
OTHER
- Journal Title
- BioChip Journal
- Volume
- 4
- Number
- 3
- Start Page
- 197
- End Page
- 203
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39541
- DOI
- 10.1007/s13206-010-4306-6
- ISSN
- 1976-0280
- Abstract
- Some whales caught incidentally or illegally in Korean waters have been introduced into the seafood market. Thus. the Korea Coast Guard (KCG) monitors and reports incidentally caught whales. However, species identification of some carcasses based on morphological features is difficult and often inaccurate, particular for processed whale products in the marketplace. For these reasons, we developed a DNA microarray that distinguishes 9 species of cetacean animals for quick and simple species identification. We verified genetic variations in whales and designed species-specific oligonucleotide probes by sequence analysis of mitochondrial cytochrome c oxidase subunit I. Fluorescent dye-labeled DNA targets were generated with a fluorescent dye-labeled primer and hybridized onto a DNA microarray consisting of short probes that were 29-37 nucleotides in length. A DNA microarray system using species-specific probes was successful at rapidly identifying 9 species of whales on the basis of hybridization patterns.
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Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > ERICA 의약생명과학과 > 1. Journal Articles
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > DEPARTMENT OF MARINE SCIENCE AND CONVERGENCE ENGINEERING > 1. Journal Articles
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