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Detection of single-molecule H2O2 signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes

Authors
Jin, HongHeller, Daniel A.Kalbacova, MarieKim, Jong-HoZhang, JingqingBoghossian, Ardemis A.Maheshri, NarendraStrano, Michael S.
Issue Date
Apr-2010
Publisher
Nature Publishing Group
Keywords
epidermal growth factor receptor; single walled nanotube; staining; hydrogen peroxide; cell membrane; human cell; atomic force microscopy; carcinoma cell; fluorescence microscopy; article; molecule; priority journal; human; stochastic model; reactive oxyg
Citation
Nature Nanotechnology, v.5, no.4, pp.302 - 309
Indexed
SCIE
SCOPUS
Journal Title
Nature Nanotechnology
Volume
5
Number
4
Start Page
302
End Page
309
URI
https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/39902
DOI
10.1038/nnano.2010.24
ISSN
1748-3387
Abstract
An emerging concept in cell signalling is the natural role of reactive oxygen species such as hydrogen peroxide (H2O2) as beneficial messengers in redox signalling pathways. The nature of H2O2 signalling is confounded, however, by difficulties in tracking it in living systems, both spatially and temporally, at low concentrations. Here, we develop an array of fluorescent single-walled carbon nanotubes that can selectively record, in real time, the discrete, stochastic quenching events that occur as H2O2 molecules are emitted from individual human epidermal carcinoma cells stimulated by epidermal growth factor. We show mathematically that such arrays can distinguish between molecules originating locally on the cell membrane from other contributions. We find that epidermal growth factor induces 2 nmol H2O2 locally over a period of 50 min. This platform promises a new approach to understanding the signalling of reactive oxygen species at the cellular level.
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Kim, Jong-Ho
ERICA 공학대학 (DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING)
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