A Bre1-associated Protein, Large 1 (Lge1), Promotes H2B Ubiquitylation during the Early Stages of Transcription Elongationopen access
- Authors
- Song, Young-Ha; Ahn, Seong Hoon
- Issue Date
- Jan-2010
- Publisher
- American Society for Biochemistry and Molecular Biology Inc.
- Keywords
- RNA-POLYMERASE-II; UBIQUITINATION; YEAST; HISTONE H2B; TERMINAL DOMAIN; REGULATES H3 METHYLATION; SAGA COMPLEX; SACCHAROMYCES-CEREVISIAE; SYSTEMATIC IDENTIFICATION; MASS-SPECTROMETRY
- Citation
- Journal of Biological Chemistry, v.285, no.4, pp.2361 - 2367
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Biological Chemistry
- Volume
- 285
- Number
- 4
- Start Page
- 2361
- End Page
- 2367
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/40025
- DOI
- 10.1074/jbc.M109.039255
- ISSN
- 0021-9258
- Abstract
- Transcription activation has been proposed to require both ubiquitylation and deubiquitylation of histone H2B. Here, we show that Lge1 (Large 1) is found in a complex containing Rad6.Bre1 and that it controls the recruitment of Bre1, a ubiquitin ligase, and Ubp8, a deubiquitylase, to promote ubiquitylation during the early steps in elongation. Chromatin immuno-precipitation experiments showed that Lge1 associates with promoter and coding regions of actively transcribed genes in a transcription-dependent manner. Disruption of Lge1 abolished ubiquitylation of histone H2B on lysine 123 and H3 methylation on lysines 4 and 79 and resulted in significant sensitivity to 6-azauracil and mycophenolic acid. In particular, in Lge1-deficient cells, Bre1 recruitment was attenuated, whereas recruitment of Ubp8 was facilitated. These alterations were coincident with changes in the interaction between Bre1.Ubp8 and RNA polymerase II phosphorylated at serine 5 of the C-terminal domain. We propose that Lge1 has a novel function in disrupting the balance between the recruitment of Bre1 and Ubp8, thus promoting transcription elongation.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > ERICA 의약생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/40025)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.