Validation of 99mTc-labeled “4+1” fatty acids for myocardial metabolism and flow imaging: Part 1: myocardial extraction and biodistribution
- Authors
- Mirtschink, Peter; Stehr, Sebastian N.; Walther, Martin; Pietzsch, Jens; Bergmann, Ralf; Pietzsch, Hans-Juergen; Weichsel, Johannes; Pexa, Annette; Dieterich, Peter; Wunderlich, Gerd; Binas, Bert; Kropp, Joachim; Deussen, Andreas
- Issue Date
- Oct-2009
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Technetium fatty acids; isolated heart; H-FABP; CD36; myocardial imaging
- Citation
- Nuclear Medicine and Biology, v.36, no.7, pp 833 - 843
- Pages
- 11
- Indexed
- SCIE
SCOPUS
- Journal Title
- Nuclear Medicine and Biology
- Volume
- 36
- Number
- 7
- Start Page
- 833
- End Page
- 843
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/40843
- DOI
- 10.1016/j.nucmedbio.2009.06.009
- ISSN
- 0969-8051
1872-9614
- Abstract
- Introduction: C-13, F-18 and I-123 fatty acids (FA) are used for myocardial imaging. Recently, our group showed that [Tc-99m]-labeled "4+1" FA are extracted into the rat and guinea pig myocardium. The present study evaluates determinants of myocardial uptake and whole body biodistribution of these FA derivatives. Methods: Studies were performed with isolated perfused hearts of Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR) with a FAT/CD36 deficiency, as well as with heart type FA binding protein knockout mice (H-FABP)(-/-) and H-FABP(+/+). Eight 4+1-Tc-99m-FA were applied for 3 min followed by 1-min washout. A mathematical model was used to analyze FA dynamics and binding to proteins. Whole-body distribution was studied in rats with and without Tween 80. In vitro fractionation studies with [Tc-99m]-FA assessed red blood cell uptake as well as association with plasma lipoproteins very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Results: Myocardial extraction was 19.0-33.0% of the infused dose in isolated WKY and 15.2-26.4% in SHR hearts. However, H-FABP(-/-) showed a marked reduction of tracer extraction [2.8+/-0.6%ID (percent injected dose) vs. 17+/-2%ID P<001]. Uptake in red blood cells (<1.2% ID) and incorporation into lipoproteins were negligible. Incubation of Tc-99m-FA with albumin reduced ventricular extraction (P<.001) into the range of established iodinated FA tracers. polyoxyethylene(20) sorbitan monooleate improved the heart-to-liver ratio in the biodistribution studies. Conclusions: Myocardial uptake of [Tc-99m]-FA 4+1 derivatives is dependent on H-FABP. These substances may therefore provide a new tool to specifically assess regional myocardial changes of H-FABP. (C) 2009 Elsevier Inc. All rights reserved.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > DEPARTMENT OF MOLECULAR & LIFE SCIENCE > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/40843)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.