Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy
- Authors
- Jun, Bong-Hyun; Noh, Mi Suk; Kim, Gunsung; Kang, Homan; Kim, Jong-Ho; Chung, Woo-Jae; Kim, Min-Soo; Kim, Yong-Kweon; Cho, Myung-Haing; Jeong, Dae Hong; Lee, Yoon-Sik
- Issue Date
- Aug-2009
- Publisher
- Academic Press
- Keywords
- Silver nanoparticles; Optically active materials; Magnetic materials; Polymeric materials; Surface-enhanced Raman scattering
- Citation
- Analytical Biochemistry, v.391, no.1, pp.24 - 30
- Indexed
- SCIE
SCOPUS
- Journal Title
- Analytical Biochemistry
- Volume
- 391
- Number
- 1
- Start Page
- 24
- End Page
- 30
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/40973
- DOI
- 10.1016/j.ab.2009.05.005
- ISSN
- 0003-2697
- Abstract
- This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8 mu m diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as Substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology. (C) 2009 Elsevier Inc. All rights reserved.
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