Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii
- Authors
- Islam, M. Nurul; Yoo, Hye Hyun; Sung, Chung-Ki; Dong, Mi-sook; Park, Young In; Jin, Changbae; Kim, Dong-Hyun
- Issue Date
- Mar-2009
- Publisher
- AOAC International
- Citation
- Journal of AOAC International, v.92, no.2, pp.375 - 381
- Indexed
- SCOPUS
- Journal Title
- Journal of AOAC International
- Volume
- 92
- Number
- 2
- Start Page
- 375
- End Page
- 381
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/41376
- DOI
- 10.1093/jaoac/92.2.375
- ISSN
- 1060-3271
- Abstract
- This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C-18 chromatographic column (150 x 4.6 mm, 3 mu m) was used with mobile phases consisting of 0.1% formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r(2) >0.99) was observed over the concentration range of 2-100 mu g/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays; the relative standard deviation (RSD) values were <= 5.37% and accuracies ranged from 97.1 to 104.9%. Recoveries of the compounds ranged from 94.2 to 104.2% with RSD values of <= 9.50%. The developed method was successfully applied to the analysis of ethanolic extracts of Rhizoma Cnidii samples. As a result, the concentrations of chlorogenic acid, ferulic acid, Z-ligustilide, and senkyunolide A were determined to be 0.84-5.35, 0.45-1.65, 0.74-4.39, and 0.32-1.14 mg/g herb, respectively. Thus, the developed method was found to be accurate and reproducible and is considered suitable for the qualitative and quantitative analysis of Rhizoma Cnidii for bioactive compounds.
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