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Protein expression pattern of murine macrophages treated with anthrax lethal toxin
- Authors
- Jung, Kyoung Hwa; Seo, Gwi Moon; Yoon, Jang Won; Park, Kyoung Sun; Kim, Ji Cheon; Kim, Seong Joo; Oh, Kwang Gun; Lee, Jung-Ho; Chai, Young Gyu
- Issue Date
- Nov-2008
- Publisher
- Elsevier BV
- Keywords
- Bacillus anthracis; Protein expression profiling; Two-dimensional polyacrylamide gel electrophoresis; MALDI-TOF; Murine macrophages; Lethal toxin
- Citation
- Biochimica et Biophysica Acta - Proteins and Proteomics, v.1784, no.11, pp.1501 - 1506
- Indexed
- SCIE
SCOPUS
- Volume
- 1784
- Number
- 11
- Start Page
- 1501
- End Page
- 1506
- ISSN
- 1570-9639
- Abstract
- Anthrax lethal toxin (LeTx; a combination of protective antigen and lethal factor) secreted by the vegetative cells of Bacillus anthracis is cytotoxic for certain macrophage cell lines. The role of LeTx in mediating these effects is complicated largely due to the difficulty in identifying and assigning functions to the affected proteins. To analyze the protein profile of murine macrophages treated with LeTx, we employed two-dimensional polyacrylamide gel electrophoresis and MALDI-TOF MS, and interpreted the peptide mass fingerprint data relying on the ProFound database. Among the differentially expressed spots, cleaved mitogen-activated protein kinase kinase 1 acting as a negative element in the signal transduction pathway, and glucose-6-phosphate dehydrogenase playing a rote in the protection of cells from hyperproduction of active oxygen were up-regulated in the LeTx-treated macrophages. (C) 2008 Elsevier B.V. All rights reserved.
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Related Researcher
- Lee, Jung-Ho
- ERICA 공학대학 (DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING)