Identification of Glutathione Conjugates of 1-Bromopentane and its Hepatotoxicity in Female BALB/c Mice
- Authors
- Lee, Sang Kyu; Lee, Dong Ju; Yoo, Hye Hyun; Kim, Ju Hyun; Seo, Young Nun; Shin, Sil; Choi, Jae Ho; Jeon, Tae Won; Kang, Mi Jeong; Jeong, Tae Cheon
- Issue Date
- Oct-2008
- Publisher
- 대한약학회
- Keywords
- 1-Bromopentane; Glutathione; Conjugate structure; Hepatotoxicity; In vivo; Mice
- Citation
- Archives of Pharmacal Research, v.31, no.10, pp.1317 - 1323
- Indexed
- SCOPUS
KCI
- Journal Title
- Archives of Pharmacal Research
- Volume
- 31
- Number
- 10
- Start Page
- 1317
- End Page
- 1323
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/42181
- DOI
- 10.1007/s12272-001-2112-3
- ISSN
- 0253-6269
- Abstract
- Halogenated organic compounds, such as 1-bromopentane (1-BPT), are used as cleaning agents, synthesis agents, or extraction solvents in the workplace. In the present study, glutathione (GSH) conjugation and hepatotoxicity induced by 1-BPT were investigated in female BALB/c mice. S-Bromopentyl GSH, S-bromopentyl cysteine, and mono-hydroxypentyl mercapturic acid were identified in liver by liquid chromatography-electrospray ionization tandem mass spectrometry. Oral treatment of mice with 1-BPT at 1500 mg/kg produced maximum GSH conjugates at 6 h after treatment. For hepatotoxicity tests, the animals were treated orally with 1-BPT at 375, 750, or 1500 mg/kg in corn oil once for a dose response study or at 1500 mg/kg for 6, 12, 24, or 48 h for a time course study. 1-BPT dose-dependently increased serum activity of ALT and AST and decreased hepatic GSH levels, peaking at 6 and 12 h after treatment. 1-BPT (750 and 1500 mg/kg) also significantly increased the hepatic content of malondialdehyde. Thus, 1-BPT could cause hepatotoxicity and depletion of GSH content by forming GSH conjugates, presenting a toxicity mechanism and potential biomarkers for low molecular weight haloalkanes.
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