Microchip-based multiplex electro-immunosensing system for the detection of cancer biomarkers
- Authors
- Ko, Yong-Jun; Maeng, Joon-Ho; Ahn, Yoomin; Hwang, Seung Yong; Cho, Nahm-Gyoo; Lee, Seoung-Hwan
- Issue Date
- Aug-2008
- Publisher
- John Wiley & Sons Ltd.
- Keywords
- cancer biomarker; immunogold silver staining; microbead-based immunoassay; microfilter-based immobilization; multiplex electro-immunosensing microchip
- Citation
- Electrophoresis, v.29, no.16, pp.3466 - 3476
- Indexed
- SCIE
SCOPUS
- Journal Title
- Electrophoresis
- Volume
- 29
- Number
- 16
- Start Page
- 3466
- End Page
- 3476
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/42270
- DOI
- 10.1002/elps.200800139
- ISSN
- 0173-0835
- Abstract
- Microfluidic-based microchips have become the focus of research interest for immunoassays and biomarker diagnostics. This is due to their aptitude for high-throughput processing, small sample volume, and short analysis times. In this paper, we describe the development of a microchip-based multiplex electro-immunosensing system for simultaneous detection of cancer biomarkers using gold nanoparticles and silver enhancer. Our microchip is composed of biocompatible poly(PDMS) and glass substrates. To fix the antibody-immobilized microbeads, we used pillar-type microfilters within a reaction chamber. An immunogold silver staining (IGSS) method was used to amplify the electrical signal that corresponded to the immunecomplex. To demonstrate this approach, we simultaneously assayed three cancer biomarkers, alpha -fetoprotein (AFP), carcinoembryonic antigen (CEA), and prostate-specific antigen (PSA) on the microchip. The electrical signal generated from the result of the immunoreaction was measured and monitored by a PC-based system. The overall assay time was reduced from 3-8 h to about 55 min when compared to conventional immunoassays. The working range of the proposed microchip was from 10-3 to 10-1 mu g/mL of the target antigen.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > ERICA 의약생명과학과 > 1. Journal Articles
- COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF MECHANICAL ENGINEERING > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/42270)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.