Application of multiplex bead array assay for Yq microdeletion analysis in infertile males
- Authors
- Yeom, Hye-Jung; Her, Young-Sun; Oh, Moon-Ju; Paul, Saswati; Park, Mi-Seon; Yeoun, Jong-Pil; Jung, Jin-Wook; Lee, Suman; Hwang, Seung Yong
- Issue Date
- Apr-2008
- Publisher
- Academic Press
- Keywords
- Infertile; Luminex; Multiplex PCR; STS markers; Y chromosome
- Citation
- Molecular and Cellular Probes, v.22, no.2, pp.76 - 82
- Indexed
- SCIE
SCOPUS
- Journal Title
- Molecular and Cellular Probes
- Volume
- 22
- Number
- 2
- Start Page
- 76
- End Page
- 82
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/42580
- DOI
- 10.1016/j.mcp.2007.06.004
- ISSN
- 0890-8508
- Abstract
- The purpose of this study was to apply the multiplex bead array as a diagnostic tool for male infertility. The multiplex bead array offers a new platform in high-throughput nucleic acid detection. Six loci, including sex-determining regions on the Y (SRY) chromosome as a control and five sequence-tagged sites (STS) in azoospermia-factor regions, were used in this system. Extracted genomic DNA from infertile male blood was used for multiplex polymerase chain reaction (PCR). After multiplex PCR using specific Cy3-labeled primer sets, the PCR product was hybridized with capture probes. A multiplexed PCR-liquid bead was arrayed for simultaneous detection using the Luminex system. This assay system correctly identified the presence or deletion of the Y chromosome. Therefore, this method provides a sensitive and high-throughput method for probing the deletion of the Y chromosome, and offers a completely new approach to male infertility screening. (C) 2007 Elsevier Ltd. All rights reserved.
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